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| | ==Mycobacterium smegmatis MbtA apo structure== | | ==Mycobacterium smegmatis MbtA apo structure== |
| - | <StructureSection load='5kei' size='340' side='right' caption='[[5kei]], [[Resolution|resolution]] 2.33Å' scene=''> | + | <StructureSection load='5kei' size='340' side='right'caption='[[5kei]], [[Resolution|resolution]] 2.33Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kei]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KEI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kei]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KEI FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mbtA, MSMEG_4516, MSMEI_4404 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.325Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kei OCA], [http://pdbe.org/5kei PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kei RCSB], [http://www.ebi.ac.uk/pdbsum/5kei PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kei ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kei OCA], [https://pdbe.org/5kei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kei RCSB], [https://www.ebi.ac.uk/pdbsum/5kei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kei ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0R0V0_MYCS2 A0R0V0_MYCS2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Mycs2]] | + | [[Category: Large Structures]] |
| - | [[Category: Blanchard, J S]] | + | [[Category: Mycolicibacterium smegmatis MC2 155]] |
| - | [[Category: Favrot, L]] | + | [[Category: Blanchard JS]] |
| - | [[Category: Vergnolle, O]] | + | [[Category: Favrot L]] |
| - | [[Category: 3-dihydroxybenzoate-amp ligase]] | + | [[Category: Vergnolle O]] |
| - | [[Category: Acetyl-coa synthetase-like]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Mycobactin]]
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| Structural highlights
Function
A0R0V0_MYCS2
Publication Abstract from PubMed
Iron is an essential element for life, but its soluble form is scarce in the environment and is rarer in the human body. Mycobacterium tuberculosis (Mtb) produces two aryl-capped siderophores, mycobactin (MBT) and carboxymycobactin (cMBT), to chelate intracellular iron. The adenylating enzyme MbtA catalyzes the first step of mycobactin biosynthesis in two half-reactions: activation of the salicylic acid as an acyl-adenylate and ligation onto the acyl carrier protein (ACP) domain of MbtB to form covalently salicylated MbtB-ACP. We report the first apo-MbtA structure from Mycobacterium smegmatis at 2.3 A. We demonstrate here that MbtA activity can be reversibly, post-translationally regulated by acetylation. Indeed the mycobacterial protein acetyltransferase (Pat), Rv0998, specifically acetylates MbtA on lysine 546, in a cAMP-dependent manner, leading to enzyme inhibition. MbtA acetylation can be reversed by the NAD+-dependant deacetyltransferase, Rv1151c (DAc). Deletion of Pat and DAc genes in Mtb revealed distinct phenotypes for strains lacking one or the other genes at low pH and limiting iron conditions. This study establishes a direct connection between the reversible acetylation system Pat/DAc and the ability of Mtb to adapt in limited iron conditions, which is critical for mycobacterial infection.
Post-Translational Acetylation of MbtA Modulates Mycobacterial Siderophore Biosynthesis.,Vergnolle O, Xu H, Tufariello JM, Favrot L, Malek AA, Jacobs WR Jr, Blanchard JS J Biol Chem. 2016 Aug 26. pii: jbc.M116.744532. PMID:27566542[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vergnolle O, Xu H, Tufariello JM, Favrot L, Malek AA, Jacobs WR Jr, Blanchard JS. Post-Translational Acetylation of MbtA Modulates Mycobacterial Siderophore Biosynthesis. J Biol Chem. 2016 Aug 26. pii: jbc.M116.744532. PMID:27566542 doi:http://dx.doi.org/10.1074/jbc.M116.744532
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