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| - | [[Image:252d.gif|left|200px]] | |
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| - | {{Structure
| + | ==CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING== |
| - | |PDB= 252d |SIZE=350|CAPTION= <scene name='initialview01'>252d</scene>, resolution 2.300Å
| + | <StructureSection load='252d' size='340' side='right'caption='[[252d]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[252d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=252D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=252D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=252d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=252d OCA], [https://pdbe.org/252d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=252d RCSB], [https://www.ebi.ac.uk/pdbsum/252d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=252d ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=252d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=252d OCA], [http://www.ebi.ac.uk/pdbsum/252d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=252d RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Neidle S]] |
| - | '''CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING'''
| + | [[Category: Nunn CM]] |
| - | | + | [[Category: Trent JO]] |
| - | | + | [[Category: Wood AA]] |
| - | ==Overview==
| + | |
| - | The structure of the B-DNA decamer d(CGCAATTGCG)2 has been determined by X-ray diffraction analysis to a resolution of 2.3 A and an R-factor of 17.7%. The decamer crystallises in the monoclinic space group C2 and packs with a crossed arrangement of helices and a unique crossing contact distinct from all other decamer structures. This is believed to be a direct result of the sequence-dependent minor groove width of the duplex. Crossed helix structures of DNA are valuable starting points for modelling studies of the Holliday junction. Two unique sites are observed at the cross-over junction where strand exchange may occur. A Holliday junction model has been constructed for each case and modelled using molecular mechanics and dynamics techniques. One of these models was found to be fully consistent with the available physical data.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 252D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=252D OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Sequence-dependent crossed helix packing in the crystal structure of a B-DNA decamer yields a detailed model for the Holliday junction., Wood AA, Nunn CM, Trent JO, Neidle S, J Mol Biol. 1997 Jun 27;269(5):827-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9223644 9223644]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Neidle, S.]] | + | |
| - | [[Category: Nunn, C M.]] | + | |
| - | [[Category: Trent, J O.]] | + | |
| - | [[Category: Wood, A A.]] | + | |
| - | [[Category: b-dna]]
| + | |
| - | [[Category: double helix]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:45:22 2008''
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