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| - | [[Image:261d.gif|left|200px]] | |
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| - | {{Structure
| + | ==CRYSTAL STRUCTURE OF THE DNA DECAMER D(CGCAATTGCG) COMPLEXED WITH THE MINOR GROOVE BINDING DRUG NETROPSIN== |
| - | |PDB= 261d |SIZE=350|CAPTION= <scene name='initialview01'>261d</scene>, resolution 2.400Å
| + | <StructureSection load='261d' size='340' side='right'caption='[[261d]], [[Resolution|resolution]] 2.40Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NT:NETROPSIN'>NT</scene>
| + | <table><tr><td colspan='2'>[[261d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=261D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=261D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NT:NETROPSIN'>NT</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=261d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=261d OCA], [https://pdbe.org/261d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=261d RCSB], [https://www.ebi.ac.uk/pdbsum/261d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=261d ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=261d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=261d OCA], [http://www.ebi.ac.uk/pdbsum/261d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=261d RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''CRYSTAL STRUCTURE OF THE DNA DECAMER D(CGCAATTGCG) COMPLEXED WITH THE MINOR GROOVE BINDING DRUG NETROPSIN'''
| + | [[Category: Garman E]] |
| - | | + | [[Category: Neidle S]] |
| - | | + | [[Category: Nunn CM]] |
| - | ==Overview==
| + | |
| - | The crystal structure of netropsin bound to the decamer d(CGCAATTGCG) has been determined at 2.4 A resolution. This is the first example of a crystal structure of netropsin bound to decamer DNA. The central eight bases of each DNA single-strand base pair with a self-complementary strand to form an octamer B-DNA duplex. These duplexes lie end to end within the unit cell. The terminal 5'-C and G-3' bases are unpaired and interact with the neighboring duplexes via interactions within both the major and minor groove to form base triplet interactions of the type C(+)-G x C and G*(G x C), respectively. The triplet interaction of the type C(+)-G x C is known to exist within triplex DNA with the C+ base oriented parallel with the Watson-Crick guanine base to which it hydrogen bonds. The netropsin molecule lies within the minor groove of the octamer duplex and assumes a class I type position, with bifurcated hydrogen-bonding interactions from the amide groups of the netropsin to the A x T base pairs of the minor groove. The netropsin molecule fits within a five base pair long minor groove site by bending of the flexible amidinium group at one end of the drug.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 261D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=261D OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structure of the DNA decamer d(CGCAATTGCG) complexed with the minor groove binding drug netropsin., Nunn CM, Garman E, Neidle S, Biochemistry. 1997 Apr 22;36(16):4792-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9125500 9125500]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Garman, E.]] | + | |
| - | [[Category: Neidle, S.]] | + | |
| - | [[Category: Nunn, C M.]] | + | |
| - | [[Category: b-dna]]
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| - | [[Category: complexed with drug]]
| + | |
| - | [[Category: continuous helix]]
| + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: flipped-out base]]
| + | |
| - | [[Category: intermolecular base triplet]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:45:34 2008''
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