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| - | [[Image:296d.gif|left|200px]] | |
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| - | {{Structure
| + | ==SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX== |
| - | |PDB= 296d |SIZE=350|CAPTION= <scene name='initialview01'>296d</scene>, resolution 2.250Å
| + | <StructureSection load='296d' size='340' side='right'caption='[[296d]], [[Resolution|resolution]] 2.25Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=HT:2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-BENZIMIDAZOLE'>HT</scene>
| + | <table><tr><td colspan='2'>[[296d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=296D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HT:2-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI-BENZIMIDAZOLE'>HT</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=296d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=296d OCA], [https://pdbe.org/296d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=296d RCSB], [https://www.ebi.ac.uk/pdbsum/296d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=296d ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=296d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=296d OCA], [http://www.ebi.ac.uk/pdbsum/296d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=296d RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX'''
| + | [[Category: Brown DG]] |
| - | | + | [[Category: Neidle S]] |
| - | | + | [[Category: Skelly JV]] |
| - | ==Overview==
| + | [[Category: Spink N]] |
| - | The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 296D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7515488 7515488]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Brown, D G.]] | + | |
| - | [[Category: Neidle, S.]] | + | |
| - | [[Category: Skelly, J V.]] | + | |
| - | [[Category: Spink, N.]] | + | |
| - | [[Category: b-dna]]
| + | |
| - | [[Category: complexed with drug]]
| + | |
| - | [[Category: double helix]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:46:01 2008''
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