5ypt
From Proteopedia
(Difference between revisions)
m (Protected "5ypt" [edit=sysop:move=sysop]) |
|||
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Marchantia paleacea chalone synthase like 1 (CHSL1)== | |
| + | <StructureSection load='5ypt' size='340' side='right'caption='[[5ypt]], [[Resolution|resolution]] 2.39Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ypt]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Marchantia_polymorpha Marchantia polymorpha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YPT FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.394Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ypt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ypt OCA], [https://pdbe.org/5ypt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ypt RCSB], [https://www.ebi.ac.uk/pdbsum/5ypt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ypt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q5I6Y2_MARPO Q5I6Y2_MARPO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chalcone synthases (CHSs) of the type III polyketide synthases (PKSs), catalyze the formation of a tetraketide intermediate from a CoA-tethered starter and malonyl-CoA but use different cyclization mechanisms to produce distinct chemical scaffolds. Herein, we characterized CHS and CHS-like enzymes (designated MpCHS and MpCHSL1, 2 and 3) from Marchantia paleacea and determined the crystal structure of MpCHSL1. MpCHS catalyzed a Claisen condensation to form chalcone, while MpCHSLs catalyzed the formation of lactonized alpha-pyrones in vitro. Based on the structural, mutational and in vitro biochemical analyses, we established that MpCHSL1 is structurally and functionally closer to prototype CHS than stilbene synthase, and characterized the structural basis for the functional diversity of the type III PKSs. A chalcone-forming mutant of MpCHSL1 was build directed by the structural information. These findings pave the way for future studies to elucidate the functional diversity of type III PKSs in liverwort. | ||
| - | + | Structural and biochemical characterization of the plant type III polyketide synthases of the liverwort Marchantia paleacea.,Yu HN, Liu XY, Gao S, Sun B, Zheng HB, Ji M, Cheng AX, Lou HX Plant Physiol Biochem. 2018 Apr;125:95-105. doi: 10.1016/j.plaphy.2018.01.030., Epub 2018 Jan 31. PMID:29428820<ref>PMID:29428820</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5ypt" style="background-color:#fffaf0;"></div> |
| - | [[Category: Lou | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Marchantia polymorpha]] | ||
| + | [[Category: Lou HX]] | ||
| + | [[Category: Yu H]] | ||
Current revision
Crystal structure of Marchantia paleacea chalone synthase like 1 (CHSL1)
| |||||||||||
