This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2a4x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:40, 13 March 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2a4x.gif|left|200px]]
 
-
{{Structure
+
==Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2==
-
|PDB= 2a4x |SIZE=350|CAPTION= <scene name='initialview01'>2a4x</scene>, resolution 1.40&Aring;
+
<StructureSection load='2a4x' size='340' side='right'caption='[[2a4x]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene>
+
<table><tr><td colspan='2'>[[2a4x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_caespitosus Streptomyces caespitosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A4X FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-
|GENE= mrd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53502 Streptomyces caespitosus])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4x OCA], [https://pdbe.org/2a4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a4x RCSB], [https://www.ebi.ac.uk/pdbsum/2a4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a4x ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4x OCA], [http://www.ebi.ac.uk/pdbsum/2a4x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a4x RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/O05205_STRLA O05205_STRLA]
-
 
+
== Evolutionary Conservation ==
-
'''Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2'''
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==Overview==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/2a4x_consurf.spt"</scriptWhenChecked>
-
Antibiotic-producing microorganisms must be protected from the lethal effect of their own antibiotic. We have previously determined the X-ray crystal structure of the bleomycin (Bm)-binding protein, designated BLMA, as a self-resistance determinant from Bm-producing Streptomyces verticillus, which suggests that the binding of the first Bm to one of two pockets formed in the BLMA homodimer induces the cooperative binding of the second Bm to the other pocket. In the present study, we noticed that the X-ray crystallographic structure of a self-resistance determinant from a mitomycin C-producing microorganism, designated MRDP, reveals similarity to the folding pattern on the BLMA, although no sequence homology exists. To clarify the hypothesis that MRDP may function as a resistance determinant to Bm, we characterized and determined the crystal structure of MRDP complexed with the Cu(II)-bound form of BmA(2) grouped into the Bm family of antibiotics. The biochemical and structural studies for Bm binding provide evidence that the first Bm binds anti-cooperatively to a pocket of MRDP with binding affinity of the nanomolar order, whereas the second Bm binds to the other pocket, which has binding affinity of the micromolar order. The invisibility of the second Bm in the structure agrees with the observation that Escherichia coli-expressing MRDP displays lower resistance to Bm than that expressing BLMA. The structure of MRDP, which is complexed with the Cu(II)-bound BmA(2), revealed that the gamma-aminopropyldimethylsulphonium moiety of the antibiotic is sandwiched between the peripheral residues of the binding pocket and that its positively charged sulphonium head is accommodated completely in the negatively charged region of the MRDP pocket. Furthermore, the Cu(II)-bound BmA(2) has a very compact structure, in which the bithiazole ring of BmA(2) is folded back to the metal-binding domain.
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
2A4X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_caespitosus Streptomyces caespitosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4X OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a4x ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
__TOC__
-
The mitomycin C (MMC)-binding protein from MMC-producing microorganisms protects from the lethal effect of bleomycin: crystallographic analysis to elucidate the binding mode of the antibiotic to the protein., Danshiitsoodol N, de Pinho CA, Matoba Y, Kumagai T, Sugiyama M, J Mol Biol. 2006 Jul 7;360(2):398-408. Epub 2006 May 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16756991 16756991]
+
</StructureSection>
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
[[Category: Streptomyces caespitosus]]
[[Category: Streptomyces caespitosus]]
-
[[Category: Danshiitsoodol, N.]]
+
[[Category: Danshiitsoodol N]]
-
[[Category: Kumagai, T.]]
+
[[Category: Kumagai T]]
-
[[Category: Matoba, Y.]]
+
[[Category: Matoba Y]]
-
[[Category: Pinho, C A.de.]]
+
[[Category: Sugiyama M]]
-
[[Category: Sugiyama, M.]]
+
[[Category: De Pinho CA]]
-
[[Category: alfa/beta protein]]
+
-
[[Category: bleomycin a2]]
+
-
[[Category: mitomycin c-binding protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:47:53 2008''
+

Current revision

Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2

PDB ID 2a4x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a4x"
Personal tools