|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal Structure of CalG3, Calicheamicin Glycostyltransferase, TDP and calicheamicin T0 bound form== | | ==Crystal Structure of CalG3, Calicheamicin Glycostyltransferase, TDP and calicheamicin T0 bound form== |
| - | <StructureSection load='3oti' size='340' side='right' caption='[[3oti]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='3oti' size='340' side='right'caption='[[3oti]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3oti]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15837 Atcc 15837]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OTI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3oti]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C0T:CALICHEAMICIN+T0'>C0T</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.597Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3d0r|3d0r]], [[3d0q|3d0q]], [[3otg|3otg]], [[3oth|3oth]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C0T:CALICHEAMICIN+T0'>C0T</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">calG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oti OCA], [https://pdbe.org/3oti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oti RCSB], [https://www.ebi.ac.uk/pdbsum/3oti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oti ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oti OCA], [http://pdbe.org/3oti PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3oti RCSB], [http://www.ebi.ac.uk/pdbsum/3oti PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3oti ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8KND7_MICEC Q8KND7_MICEC] |
| - | Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.
| + | |
| - | | + | |
| - | Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:21987796</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3oti" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 15837]] | + | [[Category: Large Structures]] |
| - | [[Category: Bingman, C A]] | + | [[Category: Micromonospora echinospora]] |
| - | [[Category: Structural genomic]] | + | [[Category: Bingman CA]] |
| - | [[Category: Chang, A]] | + | [[Category: Chang A]] |
| - | [[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
| + | [[Category: Phillips Jr GN]] |
| - | [[Category: Phillips, G N]] | + | [[Category: Singh S]] |
| - | [[Category: Singh, S]] | + | [[Category: Thorson JS]] |
| - | [[Category: Thorson, J S]] | + | |
| - | [[Category: Calicheamicin]]
| + | |
| - | [[Category: Cesg]]
| + | |
| - | [[Category: Enzyme discovery for natural product biosynthesis]]
| + | |
| - | [[Category: Glycosyltransferase]]
| + | |
| - | [[Category: Gt-b fold]]
| + | |
| - | [[Category: Natpro]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Tdp]]
| + | |
| - | [[Category: Transferase-antibiotic complex]]
| + | |
