3px7
From Proteopedia
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==Crystal Structure of covalent complex of topoisomerase 1A with substrate== | ==Crystal Structure of covalent complex of topoisomerase 1A with substrate== | ||
| - | <StructureSection load='3px7' size='340' side='right' caption='[[3px7]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='3px7' size='340' side='right'caption='[[3px7]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3px7]] is a 3 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3px7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH1 Escherichia coli DH1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PX7 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr> | |
| - | <tr id=' | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3px7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3px7 OCA], [https://pdbe.org/3px7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3px7 RCSB], [https://www.ebi.ac.uk/pdbsum/3px7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3px7 ProSAT]</span></td></tr> |
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| - | < | + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/TOP1_ECOLI TOP1_ECOLI] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.<ref>PMID:9497321</ref> <ref>PMID:10681504</ref> <ref>PMID:21482796</ref> |
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| + | ==See Also== | ||
| + | *[[Topoisomerase 3D structures|Topoisomerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Escherichia coli DH1]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Cheng | + | [[Category: Cheng B]] |
| - | [[Category: Tse-dinh | + | [[Category: Tse-dinh YC]] |
| - | [[Category: Zhang | + | [[Category: Zhang Z]] |
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Current revision
Crystal Structure of covalent complex of topoisomerase 1A with substrate
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