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| - | [[Image:2a7e.gif|left|200px]] | |
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| - | {{Structure
| + | ==On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength== |
| - | |PDB= 2a7e |SIZE=350|CAPTION= <scene name='initialview01'>2a7e</scene>, resolution 1.66Å
| + | <StructureSection load='2a7e' size='340' side='right'caption='[[2a7e]], [[Resolution|resolution]] 1.66Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[2a7e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A7E FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7e OCA], [https://pdbe.org/2a7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a7e RCSB], [https://www.ebi.ac.uk/pdbsum/2a7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7e ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7e OCA], [http://www.ebi.ac.uk/pdbsum/2a7e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a7e RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Mueller-Dieckmann C]] |
| - | '''On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength'''
| + | [[Category: Panjikar S]] |
| - | | + | [[Category: Tucker PA]] |
| - | | + | [[Category: Weiss MS]] |
| - | ==Overview==
| + | |
| - | Complete and highly redundant data sets were collected at different wavelengths between 0.80 and 2.65 A for a total of ten different protein and DNA model systems. The magnitude of the anomalous signal-to-noise ratio as assessed by the quotient R(anom)/R(r.i.m.) was found to be influenced by the data-collection wavelength and the nature of the anomalously scattering substructure. By utilizing simple empirical correlations, for instance between the estimated deltaF/F and the expected R(anom) or the data-collection wavelength and the expected R(r.i.m.), the wavelength at which the highest anomalous signal-to-noise ratio can be expected could be estimated even before the experiment. Almost independent of the nature of the anomalously scattering substructure and provided that no elemental X-ray absorption edge is nearby, this optimal wavelength is 2.1 A.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2A7E is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7E OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | On the routine use of soft X-rays in macromolecular crystallography. Part III. The optimal data-collection wavelength., Mueller-Dieckmann C, Panjikar S, Tucker PA, Weiss MS, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1263-72. Epub 2005, Aug 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16131760 16131760]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Mueller-Dieckmann, C.]] | + | |
| - | [[Category: Panjikar, S.]] | + | |
| - | [[Category: Tucker, P A.]] | + | |
| - | [[Category: Weiss, M S.]] | + | |
| - | [[Category: a-dna]]
| + | |
| - | [[Category: double helix]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:49 2008''
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