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3ta9

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==beta-Glucosidase A from the halothermophile H. orenii==
==beta-Glucosidase A from the halothermophile H. orenii==
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<StructureSection load='3ta9' size='340' side='right' caption='[[3ta9]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='3ta9' size='340' side='right'caption='[[3ta9]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ta9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Haloh Haloh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TA9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ta9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halothermothrix_orenii_H_168 Halothermothrix orenii H 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TA9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Hore_15280 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=373903 HALOH])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ta9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta9 OCA], [https://pdbe.org/3ta9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ta9 RCSB], [https://www.ebi.ac.uk/pdbsum/3ta9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ta9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ta9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta9 OCA], [http://pdbe.org/3ta9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ta9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ta9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ta9 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B8CYA8_HALOH B8CYA8_HALOH]
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The beta-glucosidase A gene (bglA) has been cloned from the halothermophilic bacterium Halothermothrix orenii and the recombinant enzyme (BglA; EC 3.2.1.21) was bacterially expressed, purified using metal ion-affinity chromatography and subsequently crystallized. Orthorhombic crystals were obtained that diffracted to a resolution limit of 3.5 A. The crystal structure with two molecules in the asymmetric unit was solved by molecular replacement using a library of known glucosidase structures. Attempts to collect higher resolution diffraction data from crystals grown under different conditions and structure refinement are currently in progress.
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Expression, purification and preliminary crystallographic analysis of the recombinant beta-glucosidase (BglA) from the halothermophile Halothermothrix orenii.,Kori LD, Hofmann A, Patel BK Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):111-3. Epub, 2010 Dec 23. PMID:21206038<ref>PMID:21206038</ref>
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==See Also==
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*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ta9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Beta-glucosidase]]
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[[Category: Halothermothrix orenii H 168]]
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[[Category: Haloh]]
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[[Category: Large Structures]]
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[[Category: Hofmann, A]]
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[[Category: Hofmann A]]
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[[Category: Kori, L D]]
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[[Category: Kori LD]]
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[[Category: Patel, B K]]
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[[Category: Patel BK]]
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[[Category: Wang, C K]]
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[[Category: Wang CK]]
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[[Category: Glucosidase]]
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[[Category: Hydrolase]]
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[[Category: Tim barrel]]
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Current revision

beta-Glucosidase A from the halothermophile H. orenii

PDB ID 3ta9

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