2a8m

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[[Image:2a8m.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Human Taspase1 (T234S mutant)==
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|PDB= 2a8m |SIZE=350|CAPTION= <scene name='initialview01'>2a8m</scene>, resolution 2.60&Aring;
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<StructureSection load='2a8m' size='340' side='right'caption='[[2a8m]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>
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<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8M FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE= C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [https://pdbe.org/2a8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [https://www.ebi.ac.uk/pdbsum/2a8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8m ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2a8i|2A8I]], [[2a8j|2A8J]], [[2a8l|2A8L]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [http://www.ebi.ac.uk/pdbsum/2a8m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
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== Evolutionary Conservation ==
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'''Crystal Structure of Human Taspase1 (T234S mutant)'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8m_consurf.spt"</scriptWhenChecked>
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Taspase1 catalyzes the proteolytic processing of the mixed lineage leukemia (MLL) nuclear protein, which is required for maintaining Hox gene expression patterns. Chromosomal translocations of the MLL gene are associated with leukemia in infants. Taspase1, a threonine aspartase, is a member of the type 2 asparaginase family, but is the only protease in this family. We report here the crystal structures of human activated Taspase1 and its proenzyme, as well as the characterization of the effects of mutations in the active site region using a newly developed fluorogenic assay. The structure of Taspase1 has significant differences from other asparaginases, especially near the active site. Mutation of the catalytic nucleophile, Thr234, abolishes autocatalytic processing in cis but does not completely block proteolysis in trans. The structure unexpectedly showed the binding of a chloride ion in the active site, and our kinetic studies confirm that chlorides ions are inhibitors of this enzyme at physiologically relevant concentrations.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2A8M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8m ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Crystal structure of human Taspase1, a crucial protease regulating the function of MLL., Khan JA, Dunn BM, Tong L, Structure. 2005 Oct;13(10):1443-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16216576 16216576]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dunn, B M.]]
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[[Category: Dunn BM]]
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[[Category: Khan, J A.]]
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[[Category: Khan JA]]
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[[Category: Tong, L.]]
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[[Category: Tong L]]
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[[Category: asparaginase]]
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[[Category: leukemia]]
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[[Category: mll]]
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[[Category: taspase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:49:22 2008''
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Current revision

Crystal Structure of Human Taspase1 (T234S mutant)

PDB ID 2a8m

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