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| | ==Mutant of claas II CPD photolyase from Methanosarcina mazei - W388F== | | ==Mutant of claas II CPD photolyase from Methanosarcina mazei - W388F== |
| - | <StructureSection load='5o86' size='340' side='right' caption='[[5o86]], [[Resolution|resolution]] 1.69Å' scene=''> | + | <StructureSection load='5o86' size='340' side='right'caption='[[5o86]], [[Resolution|resolution]] 1.69Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5o86]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Metma Metma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O86 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5o86]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei_Go1 Methanosarcina mazei Go1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O86 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.687Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MM_0852 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192952 METMA])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o86 OCA], [https://pdbe.org/5o86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o86 RCSB], [https://www.ebi.ac.uk/pdbsum/5o86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o86 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o86 OCA], [http://pdbe.org/5o86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o86 RCSB], [http://www.ebi.ac.uk/pdbsum/5o86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o86 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8PYK9_METMA Q8PYK9_METMA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Deoxyribodipyrimidine photo-lyase]] | + | [[Category: Large Structures]] |
| - | [[Category: Metma]] | + | [[Category: Methanosarcina mazei Go1]] |
| - | [[Category: Essen, L O]] | + | [[Category: Essen L-O]] |
| - | [[Category: Ignatz, E]] | + | [[Category: Ignatz E]] |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Photoreduction]]
| + | |
| Structural highlights
Function
Q8PYK9_METMA
Publication Abstract from PubMed
All light-sensitive members of the photolyase/cryptochrome family rely on FAD as catalytic cofactor. Its activity is regulated by photoreduction, a light-triggered electron transfer process from a conserved tryptophan triad to the flavin. The stability of the reduced flavin depends on available external electron donors and oxygen. In this study, we show for the class II photolyase of Methanosarcina mazei, MmCPDII, that it utilizes physiologically relevant redox cofactors NADH and NADPH for the formation of the semiquinoid FAD in a light-dependent reaction. Using redox-inert variants MmCPDII/W388F and MmCPDII/W360F we demonstrate that photoreduction by NADH and NADPH requires the class-II-specific tryptophan cascade of MmCPDII. Finally, we confirmed that mutations in the tryptophan cascade can be introduced without any substantial structural disturbances by analyzing crystal structures of MmCPDII/W388F, MmCPDII/W360F and MmCPDII/Y345F. This article is protected by copyright. All rights reserved.
Nicotinamide Adenine Dinucleotides Arrest Photoreduction of Class II DNA Photolyases in FADH State.,Ignatz E, Geisselbrecht Y, Kiontke S, Essen LO Photochem Photobiol. 2017 Aug 31. doi: 10.1111/php.12834. PMID:28858395[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ignatz E, Geisselbrecht Y, Kiontke S, Essen LO. Nicotinamide Adenine Dinucleotides Arrest Photoreduction of Class II DNA Photolyases in FADH State. Photochem Photobiol. 2017 Aug 31. doi: 10.1111/php.12834. PMID:28858395 doi:http://dx.doi.org/10.1111/php.12834
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