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2a9l

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[[Image:2a9l.gif|left|200px]]
 
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{{Structure
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==SOLUTION NMR STRUCTURE OF A SUBSTRATE FOR THE ARCHAEAL PRE-TRNA SPLICING ENDONUCLEASES: THE BULGE-HELIX-BULGE MOTIF, MINIMIZED AVERAGE STRUCTURE==
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|PDB= 2a9l |SIZE=350|CAPTION= <scene name='initialview01'>2a9l</scene>
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<StructureSection load='2a9l' size='340' side='right'caption='[[2a9l]]' scene=''>
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|SITE= <scene name='pdbsite=NUL:Enzymatic+Cleavage+Occurs+3&#39;+To+These+Residues'>NUL</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[2a9l]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A9L FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a9l OCA], [https://pdbe.org/2a9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a9l RCSB], [https://www.ebi.ac.uk/pdbsum/2a9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a9l ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1a9l|1A9L]]
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<div style="background-color:#fffaf0;">
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a9l OCA], [http://www.ebi.ac.uk/pdbsum/2a9l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a9l RCSB]</span>
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== Publication Abstract from PubMed ==
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}}
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'''SOLUTION NMR STRUCTURE OF A SUBSTRATE FOR THE ARCHAEAL PRE-TRNA SPLICING ENDONUCLEASES: THE BULGE-HELIX-BULGE MOTIF, MINIMIZED AVERAGE STRUCTURE'''
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==Overview==
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The structure of the bulge-helix-bulge motif that constitutes the intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy. The conformations of the two 3 nt bulges, where the pre-tRNA is cleaved, are stabilized by stacking interactions between bulge nucleotides and bases in the adjacent Watson-Crick helices and by a network of backbone hydrogen bonds. Both bulges are presented on the same minor groove face of the central 4 bp helix, and the overall structure has approximate two-fold symmetry, which makes it well-suited for attack by archaeal splicing endonucleases, which are symmetric dimers.
The structure of the bulge-helix-bulge motif that constitutes the intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy. The conformations of the two 3 nt bulges, where the pre-tRNA is cleaved, are stabilized by stacking interactions between bulge nucleotides and bases in the adjacent Watson-Crick helices and by a network of backbone hydrogen bonds. Both bulges are presented on the same minor groove face of the central 4 bp helix, and the overall structure has approximate two-fold symmetry, which makes it well-suited for attack by archaeal splicing endonucleases, which are symmetric dimers.
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==About this Structure==
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Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif.,Diener JL, Moore PB Mol Cell. 1998 May;1(6):883-94. PMID:9660971<ref>PMID:9660971</ref>
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2A9L is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A9L OCA].
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==Reference==
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Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif., Diener JL, Moore PB, Mol Cell. 1998 May;1(6):883-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9660971 9660971]
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[[Category: Protein complex]]
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[[Category: Diener, J L.]]
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[[Category: Moore, P B.]]
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[[Category: archaea]]
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[[Category: archaeal bulge-helix-bulge motif]]
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[[Category: nmr]]
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[[Category: pre-trna splicing]]
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[[Category: ribonucleic acid]]
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[[Category: rna structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:49:45 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2a9l" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Diener JL]]
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[[Category: Moore PB]]

Current revision

SOLUTION NMR STRUCTURE OF A SUBSTRATE FOR THE ARCHAEAL PRE-TRNA SPLICING ENDONUCLEASES: THE BULGE-HELIX-BULGE MOTIF, MINIMIZED AVERAGE STRUCTURE

PDB ID 2a9l

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