2aay

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[[Image:2aay.gif|left|200px]]
 
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{{Structure
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==EPSP synthase liganded with shikimate and glyphosate==
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|PDB= 2aay |SIZE=350|CAPTION= <scene name='initialview01'>2aay</scene>, resolution 1.55&Aring;
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<StructureSection load='2aay' size='340' side='right'caption='[[2aay]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GPJ:GLYPHOSATE'>GPJ</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>
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<table><tr><td colspan='2'>[[2aay]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AAY FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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|GENE= aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GPJ:GLYPHOSATE'>GPJ</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aay OCA], [https://pdbe.org/2aay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aay RCSB], [https://www.ebi.ac.uk/pdbsum/2aay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aay ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1g6s|1G6S]], [[1g6t|1G6T]], [[1q36|1Q36]], [[1mi4|1MI4]], [[1x8r|1X8R]], [[1x8t|1X8T]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aay OCA], [http://www.ebi.ac.uk/pdbsum/2aay PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aay RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/AROA_ECOLI AROA_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/2aay_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aay ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad-spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1.5 Angstroms resolution, revealed that binding of shikimate induces changes around the backbone of the active site, which in turn impact the efficient binding of glyphosate. The implications from these findings with respect to the design of novel glyphosate-insensitive EPSP synthase enzymes are discussed.
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'''EPSP synthase liganded with shikimate and glyphosate'''
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Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate.,Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:16225867<ref>PMID:16225867</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2aay" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad-spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1.5 Angstroms resolution, revealed that binding of shikimate induces changes around the backbone of the active site, which in turn impact the efficient binding of glyphosate. The implications from these findings with respect to the design of novel glyphosate-insensitive EPSP synthase enzymes are discussed.
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*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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2AAY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAY OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate., Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E, FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16225867 16225867]
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[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Becker, A.]]
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[[Category: Becker A]]
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[[Category: Funke, T.]]
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[[Category: Funke T]]
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[[Category: Healy, M L.]]
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[[Category: Healy ML]]
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[[Category: Priestman, M A.]]
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[[Category: Priestman MA]]
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[[Category: inside-out alpha/beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:50:17 2008''
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Current revision

EPSP synthase liganded with shikimate and glyphosate

PDB ID 2aay

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