6exv

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(New page: '''Unreleased structure''' The entry 6exv is ON HOLD Authors: Description: Category: Unreleased Structures)
Current revision (09:00, 9 April 2025) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 6exv is ON HOLD
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==Structure of mammalian RNA polymerase II elongation complex inhibited by Alpha-amanitin==
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<SX load='6exv' size='340' side='right' viewer='molstar' caption='[[6exv]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6exv]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EXV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=ILX:4,5-DIHYDROXYISOLEUCINE'>ILX</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRX:6-HYDROXYTRYPTOPHAN'>TRX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6exv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6exv OCA], [https://pdbe.org/6exv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6exv RCSB], [https://www.ebi.ac.uk/pdbsum/6exv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6exv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/I3LJR4_PIG I3LJR4_PIG] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RNA polymerase II (Pol II) is the central enzyme that transcribes eukaryotic protein-coding genes to produce mRNA. The mushroom toxin alpha-amanitin binds Pol II and inhibits transcription at the step of RNA chain elongation. Pol II from yeast binds alpha-amanitin with micromolar affinity, whereas metazoan Pol II enzymes exhibit nanomolar affinities. Here, we present the high-resolution cryo-EM structure of alpha-amanitin bound to and inhibited by its natural target, the mammalian Pol II elongation complex. The structure revealed that the toxin is located in a pocket previously identified in yeast Pol II, but forms additional contacts with metazoan-specific residues, which explain why its affinity to mammalian Pol II is ~3000 times higher than for yeast Pol II. Our work provides the structural basis for the inhibition of mammalian Pol II by the natural toxin alpha-amanitin and highlights that cryo-EM is well suited to studying interactions of a small molecule with its macromolecular target.
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Authors:
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Cryo-EM structure of a mammalian RNA polymerase II elongation complex inhibited by alpha-amanitin.,Liu X, Farnung L, Wigge C, Cramer P J Biol Chem. 2018 Mar 17. pii: RA118.002545. doi: 10.1074/jbc.RA118.002545. PMID:29550768<ref>PMID:29550768</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 6exv" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</SX>
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[[Category: Large Structures]]
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[[Category: Sus scrofa]]
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[[Category: Cramer P]]
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[[Category: Farnung L]]
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[[Category: Liu X]]
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[[Category: Wigge C]]

Current revision

Structure of mammalian RNA polymerase II elongation complex inhibited by Alpha-amanitin

6exv, resolution 3.60Å

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