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| | ==Multi-bunch pink beam serial crystallography: Phycocyanin (One chip)== | | ==Multi-bunch pink beam serial crystallography: Phycocyanin (One chip)== |
| - | <StructureSection load='5mjp' size='340' side='right' caption='[[5mjp]], [[Resolution|resolution]] 2.11Å' scene=''> | + | <StructureSection load='5mjp' size='340' side='right'caption='[[5mjp]], [[Resolution|resolution]] 2.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mjp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_(strain_bp-1) Thermosynechococcus elongatus (strain bp-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MJP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mjp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MJP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5mjm|5mjm]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mjp OCA], [https://pdbe.org/5mjp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mjp RCSB], [https://www.ebi.ac.uk/pdbsum/5mjp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mjp ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mjp OCA], [http://pdbe.org/5mjp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mjp RCSB], [http://www.ebi.ac.uk/pdbsum/5mjp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mjp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PHCA_THEEB PHCA_THEEB]] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. [[http://www.uniprot.org/uniprot/PHCB_THEEB PHCB_THEEB]] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. | + | [https://www.uniprot.org/uniprot/PHCA_THEVB PHCA_THEVB] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major phycobiliprotein in the PBS rod. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5mjp" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5mjp" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Phycocyanin|Phycocyanin]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lieske, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Meents, A]] | + | [[Category: Thermosynechococcus vestitus BP-1]] |
| - | [[Category: Oberthuer, D]] | + | [[Category: Lieske J]] |
| - | [[Category: Sarrou, I]] | + | [[Category: Meents A]] |
| - | [[Category: Srajer, V]] | + | [[Category: Oberthuer D]] |
| - | [[Category: Photosynthesis]] | + | [[Category: Sarrou I]] |
| - | [[Category: Phycocyanin]] | + | [[Category: Srajer V]] |
| - | [[Category: Pink beam]]
| + | |
| - | [[Category: Serial crystallography]]
| + | |
| Structural highlights
Function
PHCA_THEVB Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major phycobiliprotein in the PBS rod.
Publication Abstract from PubMed
Serial X-ray crystallography allows macromolecular structure determination at both X-ray free electron lasers (XFELs) and, more recently, synchrotron sources. The time resolution for serial synchrotron crystallography experiments has been limited to millisecond timescales with monochromatic beams. The polychromatic, "pink", beam provides a more than two orders of magnitude increased photon flux and hence allows accessing much shorter timescales in diffraction experiments at synchrotron sources. Here we report the structure determination of two different protein samples by merging pink-beam diffraction patterns from many crystals, each collected with a single 100 ps X-ray pulse exposure per crystal using a setup optimized for very low scattering background. In contrast to experiments with monochromatic radiation, data from only 50 crystals were required to obtain complete datasets. The high quality of the diffraction data highlights the potential of this method for studying irreversible reactions at sub-microsecond timescales using high-brightness X-ray facilities.
Pink-beam serial crystallography.,Meents A, Wiedorn MO, Srajer V, Henning R, Sarrou I, Bergtholdt J, Barthelmess M, Reinke PYA, Dierksmeyer D, Tolstikova A, Schaible S, Messerschmidt M, Ogata CM, Kissick DJ, Taft MH, Manstein DJ, Lieske J, Oberthuer D, Fischetti RF, Chapman HN Nat Commun. 2017 Nov 3;8(1):1281. doi: 10.1038/s41467-017-01417-3. PMID:29097720[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Meents A, Wiedorn MO, Srajer V, Henning R, Sarrou I, Bergtholdt J, Barthelmess M, Reinke PYA, Dierksmeyer D, Tolstikova A, Schaible S, Messerschmidt M, Ogata CM, Kissick DJ, Taft MH, Manstein DJ, Lieske J, Oberthuer D, Fischetti RF, Chapman HN. Pink-beam serial crystallography. Nat Commun. 2017 Nov 3;8(1):1281. doi: 10.1038/s41467-017-01417-3. PMID:29097720 doi:http://dx.doi.org/10.1038/s41467-017-01417-3
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