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2aeu

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[[Image:2aeu.gif|left|200px]]
 
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{{Structure
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==MJ0158, apo form==
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|PDB= 2aeu |SIZE=350|CAPTION= <scene name='initialview01'>2aeu</scene>, resolution 1.70&Aring;
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<StructureSection load='2aeu' size='340' side='right'caption='[[2aeu]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[2aeu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AEU FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE= mj0158 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aeu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aeu OCA], [https://pdbe.org/2aeu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aeu RCSB], [https://www.ebi.ac.uk/pdbsum/2aeu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aeu ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2aev|2AEV]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aeu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aeu OCA], [http://www.ebi.ac.uk/pdbsum/2aeu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aeu RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Y158_METJA Y158_METJA]
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== Evolutionary Conservation ==
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'''MJ0158, apo form'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/2aeu_consurf.spt"</scriptWhenChecked>
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Bacterial selenocysteine synthase converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) for selenoprotein biosynthesis. The identity of this enzyme in archaea and eukaryotes is unknown. On the basis of sequence similarity, a conserved open reading frame has been annotated as a selenocysteine synthase gene in archaeal genomes. We have determined the crystal structure of the corresponding protein from Methanococcus jannaschii, MJ0158. The protein was found to be dimeric with a distinctive domain arrangement and an exposed active site, built from residues of the large domain of one protomer alone. The shape of the dimer is reminiscent of a substructure of the decameric Escherichia coli selenocysteine synthase seen in electron microscopic projections. However, biochemical analyses demonstrated that MJ0158 lacked affinity for E. coli seryl-tRNA(Sec) or M. jannaschii seryl-tRNA(Sec), and neither substrate was directly converted to selenocysteinyl-tRNA(Sec) by MJ0158 when supplied with selenophosphate. We then tested a hypothetical M. jannaschii O-phosphoseryl-tRNA(Sec) kinase and demonstrated that the enzyme converts seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec) that could constitute an activated intermediate for selenocysteinyl-tRNA(Sec) production. MJ0158 also failed to convert O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec). In contrast, both archaeal and bacterial seryl-tRNA synthetases were able to charge both archaeal and bacterial tRNA(Sec) with serine, and E. coli selenocysteine synthase converted both types of seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec). These findings demonstrate that a number of factors from the selenoprotein biosynthesis machineries are cross-reactive between the bacterial and the archaeal systems but that MJ0158 either does not encode a selenocysteine synthase or requires additional factors for activity.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2AEU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEU OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aeu ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structural and functional investigation of a putative archaeal selenocysteine synthase., Kaiser JT, Gromadski K, Rother M, Engelhardt H, Rodnina MV, Wahl MC, Biochemistry. 2005 Oct 11;44(40):13315-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16201757 16201757]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Single protein]]
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[[Category: Engelhardt H]]
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[[Category: Engelhardt, H.]]
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[[Category: Gromadski K]]
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[[Category: Gromadski, K.]]
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[[Category: Kaiser JT]]
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[[Category: Kaiser, J T.]]
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[[Category: Rodnina MV]]
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[[Category: Rodnina, M V.]]
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[[Category: Rother M]]
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[[Category: Rother, M.]]
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[[Category: Wahl MC]]
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[[Category: Wahl, M C.]]
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[[Category: homo-oligomerization]]
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[[Category: plp]]
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[[Category: pyridoxal phosphate]]
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[[Category: selenocysteine synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:51:41 2008''
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Current revision

MJ0158, apo form

PDB ID 2aeu

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