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| | ==Structure of KSRP in context of Trypanosoma cruzi 40S== | | ==Structure of KSRP in context of Trypanosoma cruzi 40S== |
| - | <StructureSection load='5opt' size='340' side='right' caption='[[5opt]], [[Resolution|resolution]] 4.00Å' scene=''> | + | <SX load='5opt' size='340' side='right' viewer='molstar' caption='[[5opt]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5opt]] is a 35 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5opt]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi_strain_CL_Brener Trypanosoma cruzi strain CL Brener]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OPT FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5opt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opt OCA], [http://pdbe.org/5opt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opt RCSB], [http://www.ebi.ac.uk/pdbsum/5opt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opt ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5opt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opt OCA], [https://pdbe.org/5opt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5opt RCSB], [https://www.ebi.ac.uk/pdbsum/5opt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5opt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/Q4CQ63_TRYCC Q4CQ63_TRYCC]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.[HAMAP-Rule:MF_03015] | + | [https://www.uniprot.org/uniprot/Q4CXR4_TRYCC Q4CXR4_TRYCC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5opt" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5opt" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Bochler, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Chicher, J]] | + | [[Category: Trypanosoma cruzi strain CL Brener]] |
| - | [[Category: Hashem, Y]] | + | [[Category: Bochler A]] |
| - | [[Category: Mancera-Martinez, E]]
| + | [[Category: Brito Querido J]] |
| - | [[Category: Querido, J Brito]] | + | [[Category: Chicher J]] |
| - | [[Category: Simonetti, A]] | + | [[Category: Hashem Y]] |
| - | [[Category: Vicens, Q]] | + | [[Category: Mancera-Martinez E]] |
| - | [[Category: Cryo-em]] | + | [[Category: Simonetti A]] |
| - | [[Category: Kinetoplastid]] | + | [[Category: Vicens Q]] |
| - | [[Category: Ksrp]] | + | |
| - | [[Category: Ribosome]]
| + | |
| Structural highlights
Function
Q4CXR4_TRYCC
Publication Abstract from PubMed
Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti-kinetoplastid therapeutic molecules that can replace the scarce and highly cytotoxic current drugs. The kinetoplastid ribosome represents a potential therapeutic target due to its relative structural divergence when compared with its human counterpart. However, several kinetoplastid-specific ribosomal features remain uncharacterized. Here, we present the near-atomic cryoelectron microscopy structure of a novel bona fide kinetoplastid-specific ribosomal (r-) protein (KSRP) bound to the ribosome. KSRP is an essential protein located at the solvent face of the 40S subunit, where it binds and stabilizes kinetoplastid-specific domains of rRNA, suggesting its role in ribosome integrity. KSRP also interacts with the r-protein eS6 at a region that is only conserved in kinetoplastids. The kinetoplastid-specific ribosomal environment of KSRP provides a promising target for the design of safer anti-kinetoplastidian drugs.
The cryo-EM Structure of a Novel 40S Kinetoplastid-Specific Ribosomal Protein.,Brito Querido J, Mancera-Martinez E, Vicens Q, Bochler A, Chicher J, Simonetti A, Hashem Y Structure. 2017 Oct 13. pii: S0969-2126(17)30305-2. doi:, 10.1016/j.str.2017.09.014. PMID:29107485[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Brito Querido J, Mancera-Martinez E, Vicens Q, Bochler A, Chicher J, Simonetti A, Hashem Y. The cryo-EM Structure of a Novel 40S Kinetoplastid-Specific Ribosomal Protein. Structure. 2017 Oct 13. pii: S0969-2126(17)30305-2. doi:, 10.1016/j.str.2017.09.014. PMID:29107485 doi:http://dx.doi.org/10.1016/j.str.2017.09.014
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