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| | ==Crystal Structure of apo Acyl-CoA Carboxylase== | | ==Crystal Structure of apo Acyl-CoA Carboxylase== |
| - | <StructureSection load='4fb8' size='340' side='right' caption='[[4fb8]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='4fb8' size='340' side='right'caption='[[4fb8]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fb8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FB8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FB8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fb8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FB8 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g2r|4g2r]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">accD6, MT2307, MTCY427.28, Rv2247 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fb8 OCA], [https://pdbe.org/4fb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fb8 RCSB], [https://www.ebi.ac.uk/pdbsum/4fb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fb8 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fb8 OCA], [http://pdbe.org/4fb8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fb8 RCSB], [http://www.ebi.ac.uk/pdbsum/4fb8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fb8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/ACCD6_MYCTU ACCD6_MYCTU] Component of a biotin-dependent acyl-CoA carboxylase complex. This subunit transfers the CO2 from carboxybiotin to the CoA ester substrate (PubMed:17114269). When associated with the alpha3 subunit AccA3, is involved in the carboxylation of acetyl-CoA and propionyl-CoA, with a preference for acetyl-CoA (PubMed:17114269).<ref>PMID:17114269</ref> |
| - | In Mycobacterium tuberculosis (Mtb) the carboxylation of acetyl-CoA to produce malonyl-CoA, a building block in long chain fatty acid biosynthesis, is catalyzed by two enzymes working sequentially: a biotin carboxylase (AccA), and a carboxyltransferase (AccD). While the exact roles of the three different biotin carboxylases (AccA1-3) and the six carboxyltransferases (AccD1-6) in Mtb are still not clear, AccD6 in complex with AccA3 can synthesize malonyl-CoA from acetyl-CoA. A series of ten herbicides that target plant acetyl-CoA carboxylases (ACC) were tested for inhibition of AccD6 and for whole-cell activity against Mtb. From the tested herbicides, haloxyfop, an arylophenoxypropionate, showed in vitro inhibition of Mtb AccD6, with an IC50 = 21.4 +/- 1 muM. Here, we report the crystal structures of Mtb AccD6 in the apo form (3.0 A) and in complex with haloxyfop-R (2.3 A). The structure of Mtb AccD6 in complex with haloxyfop-R shows two molecules of the inhibitor bound on each AccD6 subunit. These results represent the potential for developing novel therapeutics for tuberculosis based on herbicides with low human toxicity.
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| - | Structure, Activity, and Inhibition of the Carboxyltransferase beta-subunit of Acetyl-CoA Carboxylase (AccD6) from Mycobacterium tuberculosis.,Reddy MC, Breda A, Bruning JB, Sherekar M, Valluru S, Thurman C, Ehrenfeld H, Sacchettini JC Antimicrob Agents Chemother. 2014 Aug 4. pii: AAC.02574-13. PMID:25092705<ref>PMID:25092705</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4fb8" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Propionyl-CoA carboxylase]] | + | [[Category: Large Structures]] |
| - | [[Category: Bruning, J B]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Ehrenfeld, H]] | + | [[Category: Bruning JB]] |
| - | [[Category: Reddy, M C.M]] | + | [[Category: Ehrenfeld H]] |
| - | [[Category: Sacchettini, J C]] | + | [[Category: Reddy MCM]] |
| - | [[Category: Sherekar, M]] | + | [[Category: Sacchettini JC]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Sherekar M]] |
| - | [[Category: Valluru, S]] | + | [[Category: Valluru S]] |
| - | [[Category: Acyl-coa carboxylase]]
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| - | [[Category: Crotonase fold]]
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| - | [[Category: Ligase]]
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| - | [[Category: PSI, Protein structure initiative]]
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| - | [[Category: Tbsgc]]
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