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4glq

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==Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain==
==Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain==
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<StructureSection load='4glq' size='340' side='right' caption='[[4glq]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
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<StructureSection load='4glq' size='340' side='right'caption='[[4glq]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4glq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GLQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4glq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GLQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=VRB:PHYCOVIOLOBILIN,+BLUE+LIGHT-ABSORBING+FORM'>VRB</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.772&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fof|4fof]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VRB:PHYCOVIOLOBILIN,+BLUE+LIGHT-ABSORBING+FORM'>VRB</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tll0569 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB]), HO1 and PcyA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4glq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4glq OCA], [https://pdbe.org/4glq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4glq RCSB], [https://www.ebi.ac.uk/pdbsum/4glq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4glq ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4glq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4glq OCA], [http://pdbe.org/4glq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4glq RCSB], [http://www.ebi.ac.uk/pdbsum/4glq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4glq ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8DLC7_THEVB Q8DLC7_THEVB]
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The phytochrome superfamily encompasses a diverse collection of photochromic photoreceptors in plants and microorganisms that employ a covalently linked bilin cradled in a cGMP-phosphodiesterase/adenylyl-cyclase/FhlA (GAF) domain to detect light. Whereas most interconvert between red- and far-red-light-absorbing states, cyanobacteria also express variants called cyanobacteriochromes (CBCRs) that modify bilin absorption to collectively perceive the entire visible spectrum. Here, we present two X-ray crystallographic structures of the GAF domain from the blue/green photochromic CBCR PixJ from Thermosynechococcus elongatus. These structures confirm the hypothesis that CBCRs variably manipulate the chromophore pi-conjugation system through isomerization and a second thioether linkage, in this case involving the bilin C10 carbon and Cys494 within a DXCF sequence characteristic of blue/green CBCRs. Biochemical studies support a mechanism for photoconversion whereby the second linkage ruptures on route to the green-light-absorbing state. Collectively, the TePixJ(GAF) models illustrate the remarkable structural and photochemical versatility among phytochromes and CBCRs in driving light perception.
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A Photo-Labile Thioether Linkage to Phycoviolobilin Provides the Foundation for the Blue/Green Photocycles in DXCF-Cyanobacteriochromes.,Burgie ES, Walker JM, Phillips GN Jr, Vierstra RD Structure. 2013 Jan 8;21(1):88-97. doi: 10.1016/j.str.2012.11.001. Epub 2012 Dec , 6. PMID:23219880<ref>PMID:23219880</ref>
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==See Also==
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*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4glq" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Theeb]]
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[[Category: Large Structures]]
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[[Category: Burgie, E S]]
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[[Category: Thermosynechococcus vestitus BP-1]]
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[[Category: Jr, G N.Phillips]]
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[[Category: Burgie ES]]
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[[Category: Vierstra, R D]]
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[[Category: Phillips Jr GN]]
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[[Category: Walker, J M]]
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[[Category: Vierstra RD]]
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[[Category: Bili-protein]]
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[[Category: Walker JM]]
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[[Category: Bilin]]
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[[Category: Cation-pi interaction]]
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[[Category: Chromophore]]
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[[Category: Cyanobacteriochrome]]
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[[Category: Gaf domain]]
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[[Category: Photoreceptor]]
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[[Category: Phycoviolobilin]]
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[[Category: Phycoviolobilin adduct]]
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[[Category: Phytochrome]]
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[[Category: Signaling protein]]
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Current revision

Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain

PDB ID 4glq

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