2ahy

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[[Image:2ahy.gif|left|200px]]
 
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{{Structure
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==Na+ complex of the NaK Channel==
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|PDB= 2ahy |SIZE=350|CAPTION= <scene name='initialview01'>2ahy</scene>, resolution 2.40&Aring;
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<StructureSection load='2ahy' size='340' side='right'caption='[[2ahy]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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<table><tr><td colspan='2'>[[2ahy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AHY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ahy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahy OCA], [https://pdbe.org/2ahy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ahy RCSB], [https://www.ebi.ac.uk/pdbsum/2ahy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahy ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2ahz|2AHZ]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahy OCA], [http://www.ebi.ac.uk/pdbsum/2ahy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ahy RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q81HW2_BACCR Q81HW2_BACCR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/2ahy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ahy ConSurf].
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<div style="clear:both"></div>
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'''Na+ complex of the NaK Channel'''
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==See Also==
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Ion selectivity is one of the basic properties that define an ion channel. Most tetrameric cation channels, which include the K+, Ca2+, Na+ and cyclic nucleotide-gated channels, probably share a similar overall architecture in their ion-conduction pore, but the structural details that determine ion selection are different. Although K+ channel selectivity has been well studied from a structural perspective, little is known about the structure of other cation channels. Here we present crystal structures of the NaK channel from Bacillus cereus, a non-selective tetrameric cation channel, in its Na+- and K+-bound states at 2.4 A and 2.8 A resolution, respectively. The NaK channel shares high sequence homology and a similar overall structure with the bacterial KcsA K+ channel, but its selectivity filter adopts a different architecture. Unlike a K+ channel selectivity filter, which contains four equivalent K+-binding sites, the selectivity filter of the NaK channel preserves the two cation-binding sites equivalent to sites 3 and 4 of a K+ channel, whereas the region corresponding to sites 1 and 2 of a K+ channel becomes a vestibule in which ions can diffuse but not bind specifically. Functional analysis using an 86Rb flux assay shows that the NaK channel can conduct both Na+ and K+ ions. We conclude that the sequence of the NaK selectivity filter resembles that of a cyclic nucleotide-gated channel and its structure may represent that of a cyclic nucleotide-gated channel pore.
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==About this Structure==
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2AHY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHY OCA].
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==Reference==
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Atomic structure of a Na+- and K+-conducting channel., Shi N, Ye S, Alam A, Chen L, Jiang Y, Nature. 2006 Mar 23;440(7083):570-4. Epub 2006 Feb 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16467789 16467789]
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[[Category: Bacillus cereus]]
[[Category: Bacillus cereus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alam, A.]]
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[[Category: Alam A]]
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[[Category: Chen, L.]]
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[[Category: Chen L]]
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[[Category: Jiang, Y.]]
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[[Category: Jiang Y]]
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[[Category: Shi, N.]]
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[[Category: Shi N]]
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[[Category: Ye, S.]]
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[[Category: Ye S]]
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[[Category: central cavity]]
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[[Category: helix bundle]]
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[[Category: inverted teepee]]
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[[Category: ion binding]]
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[[Category: tetramer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:52:56 2008''
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Current revision

Na+ complex of the NaK Channel

PDB ID 2ahy

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