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| | ==Crystal structure of cytochrome b6f in DOPG, with disordered Rieske Iron-Sulfur Protein soluble domain== | | ==Crystal structure of cytochrome b6f in DOPG, with disordered Rieske Iron-Sulfur Protein soluble domain== |
| - | <StructureSection load='4i7z' size='340' side='right' caption='[[4i7z]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='4i7z' size='340' side='right'caption='[[4i7z]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4i7z]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Mastigocladus_laminosus Mastigocladus laminosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I7Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4i7z]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mastigocladus_laminosus Mastigocladus laminosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I7Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1E2:(2S)-3-(ACETYLOXY)-2-HYDROXYPROPYL+6-DEOXY-6-SULFO-BETA-D-GLUCOPYRANOSIDE'>1E2</scene>, <scene name='pdbligand=8K6:OCTADECANE'>8K6</scene>, <scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MYS:PENTADECANE'>MYS</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene>, <scene name='pdbligand=OZ2:(2R)-3-{[(R)-{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-2-[(6Z)-TRIDEC-6-ENOYLOXY]PROPYL+(9Z)-OCTADEC-9-ENOATE'>OZ2</scene>, <scene name='pdbligand=UMQ:UNDECYL-MALTOSIDE'>UMQ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.803Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Plastoquinol--plastocyanin_reductase Plastoquinol--plastocyanin reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.9.1 1.10.9.1] </span></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1E2:(2S)-3-(ACETYLOXY)-2-HYDROXYPROPYL+6-DEOXY-6-SULFO-BETA-D-GLUCOPYRANOSIDE'>1E2</scene>, <scene name='pdbligand=8K6:OCTADECANE'>8K6</scene>, <scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MYS:PENTADECANE'>MYS</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene>, <scene name='pdbligand=OZ2:(2R)-3-{[(R)-{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-2-[(6Z)-TRIDEC-6-ENOYLOXY]PROPYL+(9Z)-OCTADEC-9-ENOATE'>OZ2</scene>, <scene name='pdbligand=UMQ:UNDECYL-MALTOSIDE'>UMQ</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i7z OCA], [http://pdbe.org/4i7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i7z RCSB], [http://www.ebi.ac.uk/pdbsum/4i7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4i7z ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i7z OCA], [https://pdbe.org/4i7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i7z RCSB], [https://www.ebi.ac.uk/pdbsum/4i7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i7z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PETM_MASLA PETM_MASLA]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. [[http://www.uniprot.org/uniprot/CYF_MASLA CYF_MASLA]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. [[http://www.uniprot.org/uniprot/PETG_MASLA PETG_MASLA]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. PetG is required for either the stability or assembly of the cytochrome b6-f complex. [[http://www.uniprot.org/uniprot/PETD_MASLA PETD_MASLA]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. [[http://www.uniprot.org/uniprot/PETN_MASLA PETN_MASLA]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. [[http://www.uniprot.org/uniprot/CYB6_MASLA CYB6_MASLA]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.[HAMAP-Rule:MF_00633] [[http://www.uniprot.org/uniprot/UCRI_MASLA UCRI_MASLA]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. [[http://www.uniprot.org/uniprot/PETL_MASLA PETL_MASLA]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. PetL is important for photoautotrophic growth as well as for electron transfer efficiency and stability of the cytochrome b6-f complex. | + | [https://www.uniprot.org/uniprot/CYB6_MASLA CYB6_MASLA] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.[HAMAP-Rule:MF_00633] |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Cytochrome b6f catalyzes quinone redox reactions within photosynthetic membranes to generate a transmembrane proton electrochemical gradient for ATP synthesis. A key step involves the transfer of an electron from the [2Fe-2S] cluster of the iron-sulfur protein (ISP) extrinsic domain to the cytochrome f heme across a distance of 26 A, which is too large for competent electron transfer but could be bridged by translation-rotation of the ISP. Here we report the first crystallographic evidence of significant motion of the ISP extrinsic domain. It is inferred that extensive crystallographic disorder of the ISP extrinsic domain indicates conformational flexibility. The ISP disorder observed in this structure, in contrast to the largely ordered ISP structure observed in the b6f complex supplemented with neutral lipids, is attributed to electrostatic interactions arising from anionic lipids.
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| - | Lipid-induced conformational changes within the cytochrome b6f complex of oxygenic photosynthesis.,Hasan SS, Stofleth JT, Yamashita E, Cramer WA Biochemistry. 2013 Apr 16;52(15):2649-54. doi: 10.1021/bi301638h. Epub 2013 Apr, 5. PMID:23514009<ref>PMID:23514009</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4i7z" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Cytochrome f|Cytochrome f]] | + | *[[Cytochrome b6|Cytochrome b6]] |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Mastigocladus laminosus]] | | [[Category: Mastigocladus laminosus]] |
| - | [[Category: Plastoquinol--plastocyanin reductase]]
| + | [[Category: Cramer WA]] |
| - | [[Category: Cramer, W A]] | + | [[Category: Hasan SS]] |
| - | [[Category: Hasan, S S]] | + | [[Category: Stofleth JT]] |
| - | [[Category: Stofleth, J T]] | + | [[Category: Yamashita E]] |
| - | [[Category: Yamashita, E]] | + | |
| - | [[Category: Cytochrome]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Photosynthesis]]
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| - | [[Category: Plastocyanin]]
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| - | [[Category: Plastoquinol:plastocyanin oxidoreductase]]
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| - | [[Category: Thylakoid membrane]]
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