2aiv

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[[Image:2aiv.gif|left|200px]]
 
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{{Structure
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==Multiple conformations in the ligand-binding site of the yeast nuclear pore targeting domain of NUP116P==
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|PDB= 2aiv |SIZE=350|CAPTION= <scene name='initialview01'>2aiv</scene>
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<StructureSection load='2aiv' size='340' side='right'caption='[[2aiv]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2aiv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AIV FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= NUP116, NSP116 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aiv OCA], [https://pdbe.org/2aiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aiv RCSB], [https://www.ebi.ac.uk/pdbsum/2aiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aiv ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aiv OCA], [http://www.ebi.ac.uk/pdbsum/2aiv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aiv RCSB]</span>
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[https://www.uniprot.org/uniprot/NU116_YEAST NU116_YEAST] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP116 plays an important role in several nuclear export and import pathways including poly(A)+ RNA, tRNA, pre-ribosome, and protein transport.<ref>PMID:8044840</ref> <ref>PMID:8524308</ref> <ref>PMID:9463388</ref> <ref>PMID:9891088</ref> <ref>PMID:10891509</ref> <ref>PMID:10952996</ref> <ref>PMID:11071906</ref> <ref>PMID:10801828</ref> <ref>PMID:11387327</ref> <ref>PMID:11689687</ref> <ref>PMID:11104765</ref> <ref>PMID:12372823</ref> <ref>PMID:12604785</ref> <ref>PMID:15039779</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/2aiv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aiv ConSurf].
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<div style="clear:both"></div>
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'''Multiple conformations in the ligand-binding site of the yeast nuclear pore targeting domain of NUP116P'''
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==See Also==
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*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
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== References ==
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==Overview==
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<references/>
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The yeast nucleoporin Nup116p plays an important role in mRNA export and protein transport. We have determined the solution structure of the C-terminal 147 residues of this protein, the region responsible for targeting the protein to the nuclear pore complex (NPC). The structure of Nup116p-C consists of a large beta-sheet sandwiched against a smaller one, flanked on both sides by alpha-helical stretches, similar to the structure of its human homolog, NUP98. In unliganded form, Nup116p-C exhibits evidence of exchange among multiple conformations, raising the intriguing possibility that it may adopt distinct conformations when bound to different partners in the NPC. We have additionally shown that a peptide from the N terminus of the nucleoporin Nup145p-C binds Nup116p-C. This previously unknown interaction may explain the unusual asymmetric localization pattern of Nup116p in the NPC. Strikingly, the exchange phenomenon observed in the unbound state is greatly reduced in the corresponding spectra of peptide-bound Nup116p-C, suggesting that the binding interaction stabilizes the domain conformation. This study offers a high resolution view of a yeast nucleoporin structural domain and may provide insights into NPC architecture and function.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2AIV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AIV OCA].
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==Reference==
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Multiple conformations in the ligand-binding site of the yeast nuclear pore-targeting domain of Nup116p., Robinson MA, Park S, Sun ZY, Silver PA, Wagner G, Hogle JM, J Biol Chem. 2005 Oct 21;280(42):35723-32. Epub 2005 Aug 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16105837 16105837]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Hogle J]]
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[[Category: Hogle, J.]]
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[[Category: Park S]]
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[[Category: Park, S.]]
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[[Category: Robinson MA]]
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[[Category: Robinson, M A.]]
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[[Category: Silver P]]
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[[Category: Silver, P.]]
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[[Category: Sun Z-YJ]]
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[[Category: Sun, Z-Y J.]]
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[[Category: Wagner G]]
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[[Category: Wagner, G.]]
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[[Category: beta sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:53:16 2008''
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Current revision

Multiple conformations in the ligand-binding site of the yeast nuclear pore targeting domain of NUP116P

PDB ID 2aiv

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