5iww

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==Crystal structure of RNA editing factor of designer PLS-type PPR/9R protein in complex with MORF9/RIP9==
==Crystal structure of RNA editing factor of designer PLS-type PPR/9R protein in complex with MORF9/RIP9==
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<StructureSection load='5iww' size='340' side='right' caption='[[5iww]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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<StructureSection load='5iww' size='340' side='right'caption='[[5iww]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5iww]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath] and [http://en.wikipedia.org/wiki/Miscellaneous_nucleic_acid Miscellaneous nucleic acid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IWW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5iww]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IWW FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5iw8|5iw8]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MORF9, RIP9, At1g11430, T23J18.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iww OCA], [https://pdbe.org/5iww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iww RCSB], [https://www.ebi.ac.uk/pdbsum/5iww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iww ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iww OCA], [http://pdbe.org/5iww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iww RCSB], [http://www.ebi.ac.uk/pdbsum/5iww PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iww ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MORF9_ARATH MORF9_ARATH]] Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).<ref>PMID:22411807</ref> <ref>PMID:23818871</ref>
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[https://www.uniprot.org/uniprot/MORF9_ARATH MORF9_ARATH] Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).<ref>PMID:22411807</ref> <ref>PMID:23818871</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RNA editing is a post-transcriptional process that modifies the genetic information on RNA molecules. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria. The PLS-type pentatricopeptide repeat (PPR) protein and the multiple organellar RNA editing factor (MORF, also known as RNA editing factor interacting protein (RIP)) are two types of key trans-acting factors involved in this process. However, how they cooperate with one another remains unclear. Here, we have characterized the interactions between a designer PLS-type PPR protein (PLS)3PPR and MORF9, and found that RNA-binding activity of (PLS)3PPR is drastically increased on MORF9 binding. We also determined the crystal structures of (PLS)3PPR, MORF9 and the (PLS)3PPR-MORF9 complex. MORF9 binding induces significant compressed conformational changes of (PLS)3PPR, revealing the molecular mechanisms by which MORF9-bound (PLS)3PPR has increased RNA-binding activity. Similarly, increased RNA-binding activity is observed for the natural PLS-type PPR protein, LPA66, in the presence of MORF9. These findings significantly expand our understanding of MORF function in plant organellar RNA editing.
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MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing.,Yan J, Zhang Q, Guan Z, Wang Q, Li L, Ruan F, Lin R, Zou T, Yin P Nat Plants. 2017 Apr 10;3:17037. doi: 10.1038/nplants.2017.37. PMID:28394309<ref>PMID:28394309</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5iww" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Arath]]
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[[Category: Arabidopsis thaliana]]
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[[Category: Miscellaneous nucleic acid]]
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[[Category: Large Structures]]
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[[Category: Guan, Z]]
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[[Category: Unidentified]]
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[[Category: Yan, J]]
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[[Category: Guan Z]]
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[[Category: Yin, P]]
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[[Category: Yan J]]
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[[Category: Zhang, Q]]
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[[Category: Yin P]]
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[[Category: Zou, T]]
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[[Category: Zhang Q]]
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[[Category: Complex]]
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[[Category: Zou T]]
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[[Category: Morf]]
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[[Category: Pentatricopeptide repeat]]
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[[Category: Pls-type]]
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[[Category: Rna binding protein]]
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Current revision

Crystal structure of RNA editing factor of designer PLS-type PPR/9R protein in complex with MORF9/RIP9

PDB ID 5iww

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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