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| | ==Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh1== | | ==Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh1== |
| - | <StructureSection load='4xzr' size='340' side='right' caption='[[4xzr]], [[Resolution|resolution]] 2.25Å' scene=''> | + | <StructureSection load='4xzr' size='340' side='right'caption='[[4xzr]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xzr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XZR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xzr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XZR FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4pvz|4pvz]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SRC1, HEH1, YML034W, YML033W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SRP1, KAP60, YNL189W, N1606 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xzr OCA], [https://pdbe.org/4xzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xzr RCSB], [https://www.ebi.ac.uk/pdbsum/4xzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xzr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xzr OCA], [http://pdbe.org/4xzr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xzr RCSB], [http://www.ebi.ac.uk/pdbsum/4xzr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xzr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SRC1_YEAST SRC1_YEAST]] Plays a role in sister chromatid separation.<ref>PMID:11754482</ref> [[http://www.uniprot.org/uniprot/IMA1_YEAST IMA1_YEAST]] Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).<ref>PMID:7565597</ref> <ref>PMID:10913188</ref> <ref>PMID:21075847</ref> | + | [https://www.uniprot.org/uniprot/SRC1_YEAST SRC1_YEAST] Plays a role in sister chromatid separation.<ref>PMID:11754482</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Targeting of ER-synthesized membrane proteins to the inner nuclear membrane (INM) has long been explained by the diffusion-retention model. However, several INM proteins contain non-classical nuclear localization signal (NLS) sequences, which, in a few instances, have been shown to promote importin alpha/beta- and Ran-dependent translocation to the INM. Here, using structural and biochemical methods, we show that yeast INM proteins Heh2 and Src1/Heh1 contain bipartite import sequences that associate intimately with the minor NLS-binding pocket of yeast importin alpha and unlike classical NLSs efficiently displace the IBB domain in the absence of importin beta. In vivo, the intimate interactions at the minor NLS-binding pocket make the h2NLS highly efficient at recruiting importin alpha at the ER and drive INM localization of endogenous Heh2. Thus, h1/h2NLSs delineate a novel class of super-potent, IBB-like membrane protein NLSs, distinct from classical NLSs found in soluble cargos and of general interest in biology.
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| - | Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2.,Lokareddy RK, Hapsari RA, van Rheenen M, Pumroy RA, Bhardwaj A, Steen A, Veenhoff LM, Cingolani G Structure. 2015 May 19. pii: S0969-2126(15)00175-6. doi:, 10.1016/j.str.2015.04.017. PMID:26051712<ref>PMID:26051712</ref>
| + | ==See Also== |
| - | | + | *[[Importin 3D structures|Importin 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4xzr" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Cingolani, G]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Lokareddy, R K]] | + | [[Category: Cingolani G]] |
| - | [[Category: Karyopherin]] | + | [[Category: Lokareddy RK]] |
| - | [[Category: Membrane protein-nuclear protein complex]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Nl]]
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| - | [[Category: Nuclear import]]
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