5k34

<StructureSection load='5k34' size='340' side='right' caption='[[5k34]], [[Resolution|resolution]] 1.15&Aring;' scene=''>

<table><tr><td colspan='2'>[[5k34]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K34 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K34 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k35|5k35]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k34 OCA], [http://pdbe.org/5k34 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k34 RCSB], [http://www.ebi.ac.uk/pdbsum/5k34 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k34 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Ankyrin B (AnkB/LegAU13) is a translocated F box effector essential for the intracellular replication of the pathogen Legionella pneumophila. AnkB co-opts a host ubiquitin ligase to decorate the pathogen-containing vacuole with K48-linked polyubiquitinated proteins and degrade host proteins as a source of energy. Here, we report that AnkB commandeers the host ubiquitin-proteasome system through mimicry of two eukaryotic protein domains. Using X-ray crystallography, we determined the 3D structure of AnkB in complex with Skp1, a component of the human SCF ubiquitination ligase. The structure confirms that AnkB contains an N-terminal F box similar to Skp2 and a C-terminal substrate-binding domain similar to eukaryotic ankyrin repeats. We identified crucial amino acids in the substrate-binding domain of AnkB and showed them to be essential for the function of AnkB in L. pneumophila intracellular proliferation. The study reveals how Legionella uses molecular mimicry to manipulate the host ubiquitination pathway and proliferate intracellularly.

 

== References ==

[[Category: Structural genomic]]

[[Category: Gehring, K]]

[[Category: Kozlov, G]]

[[Category: Wong, K]]

[[Category: Ankyrin domain]]

[[Category: Bacterial effector]]

[[Category: Protein-protein interaction]]