5kj4
From Proteopedia
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==Crystal Structure of Mouse Protocadherin-15 EC9-10== | ==Crystal Structure of Mouse Protocadherin-15 EC9-10== | ||
| - | <StructureSection load='5kj4' size='340' side='right' caption='[[5kj4]], [[Resolution|resolution]] 3.35Å' scene=''> | + | <StructureSection load='5kj4' size='340' side='right'caption='[[5kj4]], [[Resolution|resolution]] 3.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5kj4]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[5kj4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KJ4 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kj4 OCA], [https://pdbe.org/5kj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kj4 RCSB], [https://www.ebi.ac.uk/pdbsum/5kj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kj4 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Disease == | == Disease == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/PCD15_MOUSE PCD15_MOUSE] Defects in Pcdh15 are the cause of the Ames waltzer (av) phenotype. It is characterized by deafness and a balance disorder, associated with the degeneration of inner ear neuroepithelia. |
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/PCD15_MOUSE PCD15_MOUSE] Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function. |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Tip link filaments convey force and gate inner-ear hair-cell transduction channels to mediate perception of sound and head movements. Cadherin-23 and protocadherin-15 form tip links through a calcium-dependent interaction of their extracellular domains made of multiple extracellular cadherin (EC) repeats. These repeats are structurally similar, but not identical in sequence, often featuring linkers with conserved calcium-binding sites that confer mechanical strength to them. Here we present the X-ray crystal structures of human protocadherin-15 EC8-EC10 and mouse EC9-EC10, which show an EC8-9 canonical-like calcium-binding linker, and an EC9-10 calcium-free linker that alters the linear arrangement of EC repeats. Molecular dynamics simulations and small-angle X-ray scattering experiments support this non-linear conformation. Simulations also suggest that unbending of EC9-10 confers some elasticity to otherwise rigid tip links. The new structure provides a first view of protocadherin-15's non-canonical EC linkers and suggests how they may function in inner-ear mechanotransduction, with implications for other cadherins. | ||
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| + | An elastic element in the protocadherin-15 tip link of the inner ear.,Araya-Secchi R, Neel BL, Sotomayor M Nat Commun. 2016 Nov 18;7:13458. doi: 10.1038/ncomms13458. PMID:27857071<ref>PMID:27857071</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5kj4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Mus musculus]] |
| - | [[Category: | + | [[Category: Araya-Secchi R]] |
| - | + | [[Category: Sotomayor M]] | |
| - | + | ||
| - | [[Category: | + | |
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Current revision
Crystal Structure of Mouse Protocadherin-15 EC9-10
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