5kmz
From Proteopedia
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==Solution NMR structure of Tetrahymena telomerase RNA pseudoknot== | ==Solution NMR structure of Tetrahymena telomerase RNA pseudoknot== | ||
| - | <StructureSection load='5kmz' size='340' side='right' caption='[[5kmz | + | <StructureSection load='5kmz' size='340' side='right'caption='[[5kmz]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5kmz]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2n6q 2n6q]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KMZ OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5kmz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2n6q 2n6q]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KMZ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kmz OCA], [https://pdbe.org/5kmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kmz RCSB], [https://www.ebi.ac.uk/pdbsum/5kmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kmz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). We report the cryo-electron microscopy structure of Tetrahymena telomerase at ~9 angstrom resolution. In addition to seven known holoenzyme proteins, we identify two additional proteins that form a complex (TEB) with single-stranded telomere DNA-binding protein Teb1, paralogous to heterotrimeric replication protein A (RPA). The p75-p45-p19 subcomplex is identified as another RPA-related complex, CST (CTC1-STN1-TEN1). This study reveals the paths of TER in the TERT-TER-p65 catalytic core and single-stranded DNA exit; extensive subunit interactions of the TERT essential N-terminal domain, p50, and TEB; and other subunit identities and structures, including p19 and p45C crystal structures. Our findings provide structural and mechanistic insights into telomerase holoenzyme function. | ||
| - | + | ==See Also== | |
| - | + | *[[Telomerase 3D structures|Telomerase 3D structures]] | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Tetrahymena thermophila]] |
| - | [[Category: | + | [[Category: Cash DD]] |
| - | [[Category: | + | [[Category: Feigon J]] |
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Current revision
Solution NMR structure of Tetrahymena telomerase RNA pseudoknot
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