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| | ==Solution NMR structure of human LARP7 xRRM2== | | ==Solution NMR structure of human LARP7 xRRM2== |
| - | <StructureSection load='5knw' size='340' side='right' caption='[[5knw]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5knw' size='340' side='right'caption='[[5knw]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5knw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KNW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5knw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KNW FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LARP7, HDCMA18P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5knw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5knw OCA], [https://pdbe.org/5knw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5knw RCSB], [https://www.ebi.ac.uk/pdbsum/5knw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5knw ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5knw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5knw OCA], [http://pdbe.org/5knw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5knw RCSB], [http://www.ebi.ac.uk/pdbsum/5knw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5knw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[http://www.uniprot.org/uniprot/LARP7_HUMAN LARP7_HUMAN]] Microcephalic primordial dwarfism, Alazami type. The disease is caused by mutations affecting the gene represented in this entry. | + | [https://www.uniprot.org/uniprot/LARP7_HUMAN LARP7_HUMAN] Microcephalic primordial dwarfism, Alazami type. The disease is caused by mutations affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LARP7_HUMAN LARP7_HUMAN]] Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.<ref>PMID:18249148</ref> <ref>PMID:18483487</ref> | + | [https://www.uniprot.org/uniprot/LARP7_HUMAN LARP7_HUMAN] Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.<ref>PMID:18249148</ref> <ref>PMID:18483487</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Eichhorn, C D]] | + | [[Category: Large Structures]] |
| - | [[Category: Feigon, J]] | + | [[Category: Eichhorn CD]] |
| - | [[Category: Rna binding protein]] | + | [[Category: Feigon J]] |
| - | [[Category: Rrm]]
| + | |
| - | [[Category: Xrrm]]
| + | |
| Structural highlights
Disease
LARP7_HUMAN Microcephalic primordial dwarfism, Alazami type. The disease is caused by mutations affecting the gene represented in this entry.
Function
LARP7_HUMAN Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.[1] [2]
Publication Abstract from PubMed
The 7SK small nuclear ribonucleoprotein (snRNP) sequesters and inactivates the positive transcription elongation factor b (P-TEFb), an essential eukaryotic mRNA transcription factor. The human La-related protein group 7 (hLARP7) is a constitutive component of the 7SK snRNP and localizes to the 3' terminus of the 7SK long noncoding RNA. hLARP7, and in particular its C-terminal domain (CTD), is essential for 7SK RNA stability and assembly with P-TEFb. The hLARP7 N-terminal La module binds and protects the 3' end from degradation, but the structural and functional role of its CTD is unclear. We report the solution NMR structure of the hLARP7 CTD and show that this domain contains an xRRM, a class of atypical RRM first identified in the Tetrahymena thermophila telomerase LARP7 protein p65. The xRRM binds the 3' end of 7SK RNA at the top of stem-loop 4 (SL4) and interacts with both unpaired and base-paired nucleotides. This study confirms that the xRRM is general to the LARP7 family of proteins and defines the binding site for hLARP7 on the 7SK RNA, providing insight into function.
hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA.,Eichhorn CD, Chug R, Feigon J Nucleic Acids Res. 2016 Sep 26. pii: gkw833. PMID:27679474[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ He N, Jahchan NS, Hong E, Li Q, Bayfield MA, Maraia RJ, Luo K, Zhou Q. A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis. Mol Cell. 2008 Mar 14;29(5):588-99. Epub 2008 Jan 31. PMID:18249148 doi:http://dx.doi.org/S1097-2765(08)00036-1
- ↑ Markert A, Grimm M, Martinez J, Wiesner J, Meyerhans A, Meyuhas O, Sickmann A, Fischer U. The La-related protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes. EMBO Rep. 2008 Jun;9(6):569-75. Epub 2008 May 16. PMID:18483487 doi:http://dx.doi.org/embor200872
- ↑ Eichhorn CD, Chug R, Feigon J. hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA. Nucleic Acids Res. 2016 Sep 26. pii: gkw833. PMID:27679474 doi:http://dx.doi.org/10.1093/nar/gkw833
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