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| | ==Structure of Photinus pyralis Luciferase red light emitting variant== | | ==Structure of Photinus pyralis Luciferase red light emitting variant== |
| - | <StructureSection load='5kyt' size='340' side='right' caption='[[5kyt]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='5kyt' size='340' side='right'caption='[[5kyt]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_eastern_firefly Common eastern firefly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KYT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KYT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SLU:5-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL]+ADENOSINE'>SLU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.001Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SLU:5-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL]+ADENOSINE'>SLU</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kyt OCA], [http://pdbe.org/5kyt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kyt RCSB], [http://www.ebi.ac.uk/pdbsum/5kyt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kyt ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kyt OCA], [https://pdbe.org/5kyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kyt RCSB], [https://www.ebi.ac.uk/pdbsum/5kyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kyt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm. | + | [https://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY] Produces green light with a wavelength of 562 nm. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5kyt" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5kyt" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Luciferase 3D structures|Luciferase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Common eastern firefly]] | + | [[Category: Large Structures]] |
| - | [[Category: Gulick, A M]] | + | [[Category: Photinus pyralis]] |
| - | [[Category: Adenylation forming enzyme]] | + | [[Category: Gulick AM]] |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
LUCI_PHOPY Produces green light with a wavelength of 562 nm.
Publication Abstract from PubMed
Unlike the enchanting yellow-green flashes of light produced on warm summer evenings by Photinus pyralis, the most common firefly species in North America, the orange lights of Photinus scintillans are infrequently observed. These Photinus species, and likely all bioluminescent beetles, use the same substrates beetle luciferin, ATP, and oxygen to produce light. It is the structure of the particular luciferase enzyme that is the key to determining the color of the emitted light. We report here the molecular cloning of the P. scintillans luc gene and the expression and characterization of the corresponding novel recombinant luciferase enzyme. A comparison of the amino acid sequence with that of the highly similar P. pyralis enzyme and subsequent mutagenesis studies revealed that the single conservative amino acid change tyrosine to phenylalanine at position 255 accounted for the entire emission color difference. Additional mutagenesis and crystallographic studies were performed on a H-bond network, which includes the position 255 residue and 5 other stringently conserved beetle luciferase residues, that is proximal to the substrate/emitter binding site. The results are interpreted in the context of a speculative proposal that this network is key to the understanding of bioluminescence color determination. This article is protected by copyright. All rights reserved.
Cloning of the Orange Light-Producing Luciferase from Photinus scintillans - A New Proposal on how Bioluminescence Color is Determined.,Branchini BR, Southworth TL, Fontaine DM, Murtiashaw MH, McGurk A, Talukder MH, Qureshi R, Yetil D, Sundlov JA, Gulick AM Photochem Photobiol. 2016 Nov 18. doi: 10.1111/php.12671. PMID:27861940[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Branchini BR, Southworth TL, Fontaine DM, Murtiashaw MH, McGurk A, Talukder MH, Qureshi R, Yetil D, Sundlov JA, Gulick AM. Cloning of the Orange Light-Producing Luciferase from Photinus scintillans - A New Proposal on how Bioluminescence Color is Determined. Photochem Photobiol. 2016 Nov 18. doi: 10.1111/php.12671. PMID:27861940 doi:http://dx.doi.org/10.1111/php.12671
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