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| | ==The complex of Tse2 and Tsi2 from Pseudomonas aeruginosa== | | ==The complex of Tse2 and Tsi2 from Pseudomonas aeruginosa== |
| - | <StructureSection load='5ako' size='340' side='right' caption='[[5ako]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='5ako' size='340' side='right'caption='[[5ako]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ako]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AKO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ako]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AKO FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ako FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ako OCA], [http://pdbe.org/5ako PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ako RCSB], [http://www.ebi.ac.uk/pdbsum/5ako PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ako ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ako FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ako OCA], [https://pdbe.org/5ako PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ako RCSB], [https://www.ebi.ac.uk/pdbsum/5ako PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ako ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TSI2_PSEAE TSI2_PSEAE] Immunity protein that plays a role in preventing early activation of toxin Tse2. Binds to a large surface of Tse2 and thereby occludes the active site to specifically inhibits Tse2.<ref>PMID:22310046</ref> <ref>PMID:22511866</ref> <ref>PMID:26749446</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Boraston, A B]] | + | [[Category: Large Structures]] |
| - | [[Category: Nano, F E]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Robb, C S]] | + | [[Category: Boraston AB]] |
| - | [[Category: Immunity protein]] | + | [[Category: Nano FE]] |
| - | [[Category: T6ss]] | + | [[Category: Robb CS]] |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
TSI2_PSEAE Immunity protein that plays a role in preventing early activation of toxin Tse2. Binds to a large surface of Tse2 and thereby occludes the active site to specifically inhibits Tse2.[1] [2] [3]
Publication Abstract from PubMed
Tse2 is a cytoactive toxin secreted by a type six secretion apparatus of Pseudomonas aeruginosa. The Tse2 toxin naturally attacks a target in the cytoplasm of bacterial cells, and can cause toxicity if artificially introduced into eukaryotic cells. The X-ray crystal structure of the complex of Tse2 and its cognate immunity protein Tsi2 revealed a heterotetrameric structure with an extensive binding interface. Structural identity was found between Tse2 and NAD-dependent enzymes, especially ADP-ribosylating toxins, which facilitated the identification of the Tse2 active site and revealed it to be occluded upon binding the inhibitor Tsi2. The structural identity shared with NAD-dependent enzymes, including conserved catalytic residues, suggests that the mechanism of Tse2 toxicity may be NAD dependent.
The Structure of the Toxin and Type Six Secretion System Substrate Tse2 in Complex with Its Immunity Protein.,Robb CS, Robb M, Nano FE, Boraston AB Structure. 2015 Dec 28. pii: S0969-2126(15)00501-8. doi:, 10.1016/j.str.2015.11.012. PMID:26749446[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zou T, Yao X, Qin B, Zhang M, Cai L, Shang W, Svergun DI, Wang M, Cui S, Jin Q. Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded superhelical antitoxin. J Mol Biol. 2012 Apr 6;417(4):351-61. Epub 2012 Jan 30. PMID:22310046 doi:10.1016/j.jmb.2012.01.040
- ↑ Li M, Le Trong I, Carl MA, Larson ET, Chou S, De Leon JA, Dove SL, Stenkamp RE, Mougous JD. Structural Basis for Type VI Secretion Effector Recognition by a Cognate Immunity Protein. PLoS Pathog. 2012 Apr;8(4):e1002613. Epub 2012 Apr 12. PMID:22511866 doi:10.1371/journal.ppat.1002613
- ↑ Robb CS, Robb M, Nano FE, Boraston AB. The Structure of the Toxin and Type Six Secretion System Substrate Tse2 in Complex with Its Immunity Protein. Structure. 2015 Dec 28. pii: S0969-2126(15)00501-8. doi:, 10.1016/j.str.2015.11.012. PMID:26749446 doi:http://dx.doi.org/10.1016/j.str.2015.11.012
- ↑ Robb CS, Robb M, Nano FE, Boraston AB. The Structure of the Toxin and Type Six Secretion System Substrate Tse2 in Complex with Its Immunity Protein. Structure. 2015 Dec 28. pii: S0969-2126(15)00501-8. doi:, 10.1016/j.str.2015.11.012. PMID:26749446 doi:http://dx.doi.org/10.1016/j.str.2015.11.012
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