1ojl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:01, 9 May 2024) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ojl.gif|left|200px]]<br />
 
-
<applet load="1ojl" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ojl, resolution 3.0&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF A SIGMA54-ACTIVATOR SUGGESTS THE MECHANISM FOR THE CONFORMATIONAL SWITCH NECESSARY FOR SIGMA54 BINDING'''<br />
 
-
==Overview==
+
==Crystal structure of a sigma54-activator suggests the mechanism for the conformational switch necessary for sigma54 binding==
-
The sigma(54)-dependent transcription in bacteria is associated with, various stress and growth conditions. Activators of the sigma(54) protein, contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both, prokaryotic and eukaryotic cells. We describe the X-ray structure of an, N-terminal domain deletion of the ZraR protein from Salmonella, typhimurium, which is a homologue of the general nitrogen regulatory, protein NtrC, at 3A resolution. The structure reveals a hexameric ring, that is typical for AAA+ containing proteins but which differs from the, heptameric ring found in the crystal structure of the AAA+ domain of NtrC1, from Aquifex aeolicus. The dimerisation interface between DNA-binding, domains observed in the crystal structure suggests that dodecamers, rather, than hexamers, might be the functionally important oligomer.
+
<StructureSection load='1ojl' size='340' side='right'caption='[[1ojl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ojl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OJL FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ojl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ojl OCA], [https://pdbe.org/1ojl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ojl RCSB], [https://www.ebi.ac.uk/pdbsum/1ojl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ojl ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ZRAR_SALTY ZRAR_SALTY] Member of the two-component regulatory system ZraS/ZraR. When activated by ZraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oj/1ojl_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ojl ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The sigma(54)-dependent transcription in bacteria is associated with various stress and growth conditions. Activators of the sigma(54) protein contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both prokaryotic and eukaryotic cells. We describe the X-ray structure of an N-terminal domain deletion of the ZraR protein from Salmonella typhimurium, which is a homologue of the general nitrogen regulatory protein NtrC, at 3A resolution. The structure reveals a hexameric ring that is typical for AAA+ containing proteins but which differs from the heptameric ring found in the crystal structure of the AAA+ domain of NtrC1 from Aquifex aeolicus. The dimerisation interface between DNA-binding domains observed in the crystal structure suggests that dodecamers, rather than hexamers, might be the functionally important oligomer.
-
==About this Structure==
+
Crystal structure of the central and C-terminal domain of the sigma(54)-activator ZraR.,Sallai L, Tucker PA J Struct Biol. 2005 Aug;151(2):160-70. PMID:16005641<ref>PMID:16005641</ref>
-
1OJL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with PO4 and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OJL OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure of the central and C-terminal domain of the sigma(54)-activator ZraR., Sallai L, Tucker PA, J Struct Biol. 2005 Aug;151(2):160-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16005641 16005641]
+
</div>
-
[[Category: Salmonella typhimurium]]
+
<div class="pdbe-citations 1ojl" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
== References ==
-
[[Category: Sallai, L.]]
+
<references/>
-
[[Category: Tucker, P.A.]]
+
__TOC__
-
[[Category: ATP]]
+
</StructureSection>
-
[[Category: PO4]]
+
[[Category: Large Structures]]
-
[[Category: aaa domain]]
+
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-
[[Category: dna-binding]]
+
[[Category: Sallai L]]
-
[[Category: ntrc family]]
+
[[Category: Tucker PA]]
-
[[Category: response regulator]]
+
-
[[Category: transcription regulation]]
+
-
[[Category: two component system]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 16:56:49 2007''
+

Current revision

Crystal structure of a sigma54-activator suggests the mechanism for the conformational switch necessary for sigma54 binding

PDB ID 1ojl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ojl"
Personal tools