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| - | [[Image:2awe.gif|left|200px]] | |
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| - | {{Structure
| + | ==Base-Tetrad Swapping Results in Dimerization of RNA Quadruplexes: Implications for Formation of I-Motif RNA Octaplex== |
| - | |PDB= 2awe |SIZE=350|CAPTION= <scene name='initialview01'>2awe</scene>, resolution 2.10Å
| + | <StructureSection load='2awe' size='340' side='right'caption='[[2awe]], [[Resolution|resolution]] 2.10Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=BGM:8-BROMO-2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>BGM</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[2awe]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AWE FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGM:8-BROMO-2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>BGM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2awe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2awe OCA], [https://pdbe.org/2awe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2awe RCSB], [https://www.ebi.ac.uk/pdbsum/2awe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2awe ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2awe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2awe OCA], [http://www.ebi.ac.uk/pdbsum/2awe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2awe RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''Base-Tetrad Swapping Results in Dimerization of RNA Quadruplexes: Implications for Formation of I-Motif RNA Octaplex'''
| + | [[Category: Pan B]] |
| - | | + | [[Category: Shi K]] |
| - | | + | [[Category: Sundaralingam M]] |
| - | ==Overview==
| + | |
| - | Nucleic acids adopt different multistranded helical architectures to perform various biological functions. Here, we report a crystal structure of an RNA quadruplex containing "base-tetrad swapping" and bulged nucleotide at 2.1-Angstroms resolution. The base-tetrad swapping results in a dimer of quadruplexes with an intercalated octaplex fragment at the 5' end junction. The intercalated base tetrads provide the basic repeat unit for constructing a model of intercalated RNA octaplex. The model we obtained shows fundamentally different characteristics from duplex, triplex, and quadruplex. We also observed two different orientations of bulged uridine residues that are related to the interaction with surroundings. This structural evidence reflects the conformational flexibility of bulged nucleotides in RNA quadruplexes and implies the potential roles of bulged nucleotides as recognition and interaction sites in RNA-protein and RNA-RNA interactions.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2AWE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AWE OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Base-tetrad swapping results in dimerization of RNA quadruplexes: implications for formation of the i-motif RNA octaplex., Pan B, Shi K, Sundaralingam M, Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3130-4. Epub 2006 Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16492787 16492787]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Pan, B.]] | + | |
| - | [[Category: Shi, K.]] | + | |
| - | [[Category: Sundaralingam, M.]] | + | |
| - | [[Category: octaplex]]
| + | |
| - | [[Category: rna]]
| + | |
| - | [[Category: tetraplex]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:58:09 2008''
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