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| | ==Nodal/BMP2 chimera NB250== | | ==Nodal/BMP2 chimera NB250== |
| - | <StructureSection load='4n1d' size='340' side='right' caption='[[4n1d]], [[Resolution|resolution]] 1.91Å' scene=''> | + | <StructureSection load='4n1d' size='340' side='right'caption='[[4n1d]], [[Resolution|resolution]] 1.91Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4n1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N1D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4n1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N1D FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BMP2, BMP2A, Nodal ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.912Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n1d OCA], [http://pdbe.org/4n1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n1d RCSB], [http://www.ebi.ac.uk/pdbsum/4n1d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n1d ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n1d OCA], [https://pdbe.org/4n1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n1d RCSB], [https://www.ebi.ac.uk/pdbsum/4n1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n1d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Disease == |
| | + | [https://www.uniprot.org/uniprot/NODAL_HUMAN NODAL_HUMAN] Alobar holoprosencephaly;Situs ambiguus;Lobar holoprosencephaly;Semilobar holoprosencephaly;Midline interhemispheric variant of holoprosencephaly;Septopreoptic holoprosencephaly;Situs inversus totalis;Microform holoprosencephaly. The disease is caused by variants affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BMP2_HUMAN BMP2_HUMAN]] Induces cartilage and bone formation. | + | [https://www.uniprot.org/uniprot/NODAL_HUMAN NODAL_HUMAN] Essential for mesoderm formation and axial patterning during embryonic development.[https://www.uniprot.org/uniprot/BMP2_HUMAN BMP2_HUMAN] Induces cartilage and bone formation. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4n1d" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4n1d" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Bone morphogenetic protein 3D structures|Bone morphogenetic protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Esquivies, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Cytokine]] | + | [[Category: Esquivies L]] |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Disease
NODAL_HUMAN Alobar holoprosencephaly;Situs ambiguus;Lobar holoprosencephaly;Semilobar holoprosencephaly;Midline interhemispheric variant of holoprosencephaly;Septopreoptic holoprosencephaly;Situs inversus totalis;Microform holoprosencephaly. The disease is caused by variants affecting the gene represented in this entry.
Function
NODAL_HUMAN Essential for mesoderm formation and axial patterning during embryonic development.BMP2_HUMAN Induces cartilage and bone formation.
Publication Abstract from PubMed
Nodal, a member of the TGF-beta superfamily, plays an important role in vertebrate and invertebrate early development. The biochemical study of Nodal and its signaling pathway has been a challenge, mainly because of difficulties in producing the protein in sufficient quantities. We have developed a library of stable, chemically refoldable Nodal/BMP2 chimeric ligands (NB2 library). Three chimeras, named NB250, NB260, and NB264, show Nodal-like signaling properties including dependence on the co-receptor Cripto and activation of the Smad2 pathway. NB250, like Nodal, alters heart looping during the establishment of embryonic left-right asymmetry, and both NB250 and NB260, as well as Nodal, induce chondrogenic differentiation of human adipose-derived stem cells. This Nodal-induced differentiation is shown to be more efficient than BPM2-induced differentiation. Interestingly, the crystal structure of NB250 shows a backbone scaffold similar to that of BMP2. Our results show that these chimeric ligands may have therapeutic implications in cartilage injuries.
Designer Nodal/BMP2 Chimeras Mimic Nodal Signaling, Promote Chondrogenesis, and Reveal a BMP2-like Structure.,Esquivies L, Blackler A, Peran M, Rodriguez-Esteban C, Izpisua Belmonte JC, Booker E, Gray PC, Ahn C, Kwiatkowski W, Choe S J Biol Chem. 2014 Jan 17;289(3):1788-97. doi: 10.1074/jbc.M113.529180. Epub 2013 , Dec 5. PMID:24311780[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Esquivies L, Blackler A, Peran M, Rodriguez-Esteban C, Izpisua Belmonte JC, Booker E, Gray PC, Ahn C, Kwiatkowski W, Choe S. Designer Nodal/BMP2 Chimeras Mimic Nodal Signaling, Promote Chondrogenesis, and Reveal a BMP2-like Structure. J Biol Chem. 2014 Jan 17;289(3):1788-97. doi: 10.1074/jbc.M113.529180. Epub 2013 , Dec 5. PMID:24311780 doi:http://dx.doi.org/10.1074/jbc.M113.529180
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