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5u76

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==Chicken Slo2.2 in a closed conformation vitrified in the presence of 300 mM NaCl==
==Chicken Slo2.2 in a closed conformation vitrified in the presence of 300 mM NaCl==
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<StructureSection load='5u76' size='340' side='right' caption='[[5u76]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
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<SX load='5u76' size='340' side='right' viewer='molstar' caption='[[5u76]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5u76]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U76 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5u76]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U76 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u70|5u70]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KCNT1, SLACK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u76 OCA], [https://pdbe.org/5u76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u76 RCSB], [https://www.ebi.ac.uk/pdbsum/5u76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u76 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u76 OCA], [http://pdbe.org/5u76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u76 RCSB], [http://www.ebi.ac.uk/pdbsum/5u76 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u76 ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/KCNT1_CHICK KCNT1_CHICK]] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium.
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[https://www.uniprot.org/uniprot/KCNT1_CHICK KCNT1_CHICK] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The stable structural conformations that occur along the complete reaction coordinate for ion channel opening have never been observed. In this study, we describe the equilibrium ensemble of structures of Slo2.2, a neuronal Na+-activated K+ channel, as a function of the Na+ concentration. We find that Slo2.2 exists in multiple closed conformations whose relative occupancies are independent of Na+ concentration. An open conformation emerges from an ensemble of closed conformations in a highly Na+-dependent manner, without evidence of Na+-dependent intermediates. In other words, channel opening is a highly concerted, switch-like process. The midpoint of the structural titration matches that of the functional titration. A maximum open conformation probability approaching 1.0 and maximum functional open probability approaching 0.7 imply that, within the class of open channels, there is a subclass that is not permeable to ions.
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Structural Titration of Slo2.2, a Na+-Dependent K+ Channel.,Hite RK, MacKinnon R Cell. 2017 Jan 26;168(3):390-399.e11. doi: 10.1016/j.cell.2016.12.030. Epub 2017 , Jan 19. PMID:28111072<ref>PMID:28111072</ref>
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==See Also==
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5u76" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Chick]]
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[[Category: Gallus gallus]]
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[[Category: Hite, R K]]
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[[Category: Large Structures]]
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[[Category: MacKinnon, R]]
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[[Category: Hite RK]]
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[[Category: Ion channel]]
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[[Category: MacKinnon R]]
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[[Category: Potassium channel]]
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[[Category: Transport protein]]
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Current revision

Chicken Slo2.2 in a closed conformation vitrified in the presence of 300 mM NaCl

5u76, resolution 4.20Å

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