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| | ==R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin== | | ==R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin== |
| - | <StructureSection load='5m2s' size='340' side='right' caption='[[5m2s]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='5m2s' size='340' side='right'caption='[[5m2s]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5m2s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19208 Atcc 19208] and [http://en.wikipedia.org/wiki/Ruminococcus_flavefaciens_fd-1 Ruminococcus flavefaciens fd-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M2S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5m2s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens] and [https://en.wikipedia.org/wiki/Ruminococcus_flavefaciens_FD-1 Ruminococcus flavefaciens FD-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M2S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5m2o|5m2o]], [[5aoz|5aoz]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">scaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 ATCC 19208]), glycoside hydrolase family 9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=641112 Ruminococcus flavefaciens FD-1])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m2s OCA], [https://pdbe.org/5m2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m2s RCSB], [https://www.ebi.ac.uk/pdbsum/5m2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m2s ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m2s OCA], [http://pdbe.org/5m2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m2s RCSB], [http://www.ebi.ac.uk/pdbsum/5m2s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m2s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0AEF3_RUMFL A0AEF3_RUMFL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 19208]] | + | [[Category: Large Structures]] |
| - | [[Category: Ruminococcus flavefaciens fd-1]] | + | [[Category: Ruminococcus flavefaciens]] |
| - | [[Category: Bule, P]] | + | [[Category: Ruminococcus flavefaciens FD-1]] |
| - | [[Category: Carvalho, A L]] | + | [[Category: Bule P]] |
| - | [[Category: Fontes, C M.G A]] | + | [[Category: Carvalho AL]] |
| - | [[Category: Najmudin, S]] | + | [[Category: Fontes CMGA]] |
| - | [[Category: Cell adhesion]]
| + | [[Category: Najmudin S]] |
| - | [[Category: Cellulosome]]
| + | |
| - | [[Category: Cohesin]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Dockerin]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: R. flavefacien]]
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| Structural highlights
Function
A0AEF3_RUMFL
Publication Abstract from PubMed
ABTRACT: Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates. Cellulosome assembly involves the binding of enzyme-borne dockerins (Doc) to repeated cohesin (Coh) modules located in a non-catalytic scaffoldin. Docs appended to cellulosomal enzymes generally present two similar Coh-binding interfaces supporting a dual-binding mode, which may confer increased positional adjustment of the different complex components. Ruminococcus flavefaciens' cellulosome is assembled from a repertoire of 223 Doc-containing proteins classified into 6 groups. Recent studies revealed that Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an adaptor scaffoldin. To investigate the extent to which the single-binding mode contributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to Cohs of primary (ScaA) and adaptor (ScaB) scaffoldins were solved. The data revealed that group 1 Docs display a conserved mechanism of Coh recognition involving a single-binding mode. Therefore, in contrast to all cellulosomes described to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Docs. Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous implication of the dual-binding mode in the acquisition of cellulosome flexibility.
Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes.,Bule P, Alves VD, Israeli-Ruimy V, Carvalho AL, Ferreira LM, Smith SP, Gilbert HJ, Najmudin S, Bayer EA, Fontes CM Sci Rep. 2017 Apr 7;7(1):759. doi: 10.1038/s41598-017-00919-w. PMID:28389644[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bule P, Alves VD, Israeli-Ruimy V, Carvalho AL, Ferreira LM, Smith SP, Gilbert HJ, Najmudin S, Bayer EA, Fontes CM. Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes. Sci Rep. 2017 Apr 7;7(1):759. doi: 10.1038/s41598-017-00919-w. PMID:28389644 doi:http://dx.doi.org/10.1038/s41598-017-00919-w
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