Structural highlights
Function
FGE_THECD Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins. 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile.[1] [2]
Publication Abstract from PubMed
The formylglycine-generating enzyme (FGE) is a unique copper protein that catalyzes oxygen-dependent C-H activation. We describe 1.66 A- and 1.28 A-resolution crystal structures of FGE from Thermomonospora curvata in complex with either AgI or CdII providing definitive evidence for a high-affinity metal-binding site in this enzyme. The structures reveal a bis-cysteine linear coordination of the monovalent metal, and tetrahedral coordination of the bivalent metal. Similar coordination changes may occur in the active enzyme as a result of CuI/II redox cycling. Complexation of copper atoms by two cysteine residues is common among copper-trafficking proteins, but is unprecedented for redox-active copper enzymes or synthetic copper catalysts.
Structural Basis for Copper-Oxygen Mediated C-H Bond Activation by the Formylglycine-Generating Enzyme.,Meury M, Knop M, Seebeck FP Angew Chem Int Ed Engl. 2017 Jul 3;56(28):8115-8119. doi: 10.1002/anie.201702901., Epub 2017 Jun 9. PMID:28544744[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Knop M, Engi P, Lemnaru R, Seebeck FP. In Vitro Reconstitution of Formylglycine-Generating Enzymes Requires Copper(I). Chembiochem. 2015 Oct 12;16(15):2147-50. doi: 10.1002/cbic.201500322. Epub 2015, Sep 25. PMID:26403223 doi:http://dx.doi.org/10.1002/cbic.201500322
- ↑ Knop M, Dang TQ, Jeschke G, Seebeck FP. Copper is a Cofactor of the Formylglycine-Generating Enzyme. Chembiochem. 2017 Jan 17;18(2):161-165. doi: 10.1002/cbic.201600359. Epub 2016, Dec 13. PMID:27862795 doi:http://dx.doi.org/10.1002/cbic.201600359
- ↑ Meury M, Knop M, Seebeck FP. Structural Basis for Copper-Oxygen Mediated C-H Bond Activation by the Formylglycine-Generating Enzyme. Angew Chem Int Ed Engl. 2017 Jul 3;56(28):8115-8119. doi: 10.1002/anie.201702901., Epub 2017 Jun 9. PMID:28544744 doi:http://dx.doi.org/10.1002/anie.201702901
