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5i2t

From Proteopedia

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Current revision

Domain characterization of the WD protein Pwp2 and their relevance in ribosome biogenesis

Structural highlights

5i2t is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.543Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PWP2_YEAST Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.^[1] ^[2]

Publication Abstract from PubMed

The SSU processome constitutes a large ribonucleoprotein complex involved in the early steps of ribosome biogenesis. UTP-B is one of the first multi-subunit protein complexes that associates with the pre-ribosomal RNA to form the SSU processome. To understand the molecular basis of the hierarchical assembly of the SSU-processome, we have undergone a structural and functional analysis of the UTP-B subunit Pwp2p. We show that Pwp2p is required for the proper assembly of UTP-B and for a productive association of UTP-B with pre-rRNA. These two functions are mediated by two distinct structural domains. The N-terminal domain of Pwp2p folds into a tandem WD-repeat (tWD) that associates with Utp21p, Utp18p, and Utp6p to form a core complex. The CTDs of Pwp2p and Utp21p mediate the assembly of the heterodimer Utp12p:Utp13p that is required for the stable incorporation of the UTP-B complex in the SSU processome. Finally, we provide evidence suggesting a role of UTP-B as a platform for the binding of assembly factors during the maturation of 20S rRNA precursors.

Pwp2 mediates UTP-B assembly via two structurally independent domains.,Boissier F, Schmidt CM, Linnemann J, Fribourg S, Perez-Fernandez J Sci Rep. 2017 Jun 9;7(1):3169. doi: 10.1038/s41598-017-03034-y. PMID:28600509^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dragon F, Gallagher JE, Compagnone-Post PA, Mitchell BM, Porwancher KA, Wehner KA, Wormsley S, Settlage RE, Shabanowitz J, Osheim Y, Beyer AL, Hunt DF, Baserga SJ. A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature. 2002 Jun 27;417(6892):967-70. Epub 2002 Jun 9. PMID:12068309 doi:http://dx.doi.org/10.1038/nature00769
↑ Shafaatian R, Payton MA, Reid JD. PWP2, a member of the WD-repeat family of proteins, is an essential Saccharomyces cerevisiae gene involved in cell separation. Mol Gen Genet. 1996 Aug 27;252(1-2):101-14. PMID:8804409
↑ Boissier F, Schmidt CM, Linnemann J, Fribourg S, Perez-Fernandez J. Pwp2 mediates UTP-B assembly via two structurally independent domains. Sci Rep. 2017 Jun 9;7(1):3169. doi: 10.1038/s41598-017-03034-y. PMID:28600509 doi:http://dx.doi.org/10.1038/s41598-017-03034-y

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5i2t"

Categories: Large Structures | Saccharomyces cerevisiae | Boissier F | Fribourg S

@@ Line 1: / Line 1: @@
 ==Domain characterization of the WD protein Pwp2 and their relevance in ribosome biogenesis==
-<StructureSection load='5i2t' size='340' side='right' caption='[[5i2t]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
+<StructureSection load='5i2t' size='340' side='right'caption='[[5i2t]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5i2t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I2T FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5i2t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I2T FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.543&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PWP2, UTP1, YCR057C, YCR55C/YCR57C/YCR58C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i2t OCA], [http://pdbe.org/5i2t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i2t RCSB], [http://www.ebi.ac.uk/pdbsum/5i2t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i2t ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i2t OCA], [https://pdbe.org/5i2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i2t RCSB], [https://www.ebi.ac.uk/pdbsum/5i2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i2t ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PWP2_YEAST PWP2_YEAST]] Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.<ref>PMID:12068309</ref> <ref>PMID:8804409</ref>
+[https://www.uniprot.org/uniprot/PWP2_YEAST PWP2_YEAST] Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.<ref>PMID:12068309</ref> <ref>PMID:8804409</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 18824]]
+[[Category: Large Structures]]
-[[Category: Boissier, F]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Fribourg, S]]
+[[Category: Boissier F]]
-[[Category: Biosynthetic protein]]
+[[Category: Fribourg S]]
-[[Category: Ribosome biogenesis]]
-[[Category: Wd]]

5i2t

From Proteopedia

Current revision

Domain characterization of the WD protein Pwp2 and their relevance in ribosome biogenesis

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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