|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of Lgd-Shrub complex, single chain fusion== | | ==Crystal structure of Lgd-Shrub complex, single chain fusion== |
| - | <StructureSection load='5vo5' size='340' side='right' caption='[[5vo5]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='5vo5' size='340' side='right'caption='[[5vo5]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vo5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VO5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vo5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VO5 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">shrb, Vps32, CG8055, Dmel_CG8055 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.004Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vo5 OCA], [http://pdbe.org/5vo5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vo5 RCSB], [http://www.ebi.ac.uk/pdbsum/5vo5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vo5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vo5 OCA], [https://pdbe.org/5vo5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vo5 RCSB], [https://www.ebi.ac.uk/pdbsum/5vo5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vo5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/C2D1_DROME C2D1_DROME]] Negative regulator of the Notch signaling pathway, acting to restrict the activity of Notch to the dorsoventral (D/V) boundary of the wing imaginal disk. Also causes negative regulation of Notch during vein, eye, and bristle development. Acts by targeting Notch for endosomal degradation or recycling.<ref>PMID:12648493</ref> <ref>PMID:17088062</ref> | + | [https://www.uniprot.org/uniprot/C2D1_DROME C2D1_DROME] Negative regulator of the Notch signaling pathway, acting to restrict the activity of Notch to the dorsoventral (D/V) boundary of the wing imaginal disk. Also causes negative regulation of Notch during vein, eye, and bristle development. Acts by targeting Notch for endosomal degradation or recycling.<ref>PMID:12648493</ref> <ref>PMID:17088062</ref> [https://www.uniprot.org/uniprot/Q8T0Q4_DROME Q8T0Q4_DROME] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Blacklow, S C]] | + | [[Category: Large Structures]] |
| - | [[Category: McMillan, B J]] | + | [[Category: Blacklow SC]] |
| - | [[Category: Seegar, T C.M]] | + | [[Category: McMillan BJ]] |
| - | [[Category: Escrt]] | + | [[Category: Seegar TCM]] |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
C2D1_DROME Negative regulator of the Notch signaling pathway, acting to restrict the activity of Notch to the dorsoventral (D/V) boundary of the wing imaginal disk. Also causes negative regulation of Notch during vein, eye, and bristle development. Acts by targeting Notch for endosomal degradation or recycling.[1] [2] Q8T0Q4_DROME
Publication Abstract from PubMed
The ESCRT-III complex induces outward membrane budding and fission through homotypic polymerization of its core component Shrub/CHMP4B. Shrub activity is regulated by its direct interaction with a protein called Lgd in flies, or CC2D1A or B in humans. Here, we report the structural basis for this interaction and propose a mechanism for regulation of polymer assembly. The isolated third DM14 repeat of Lgd binds Shrub, and an Lgd fragment containing only this DM14 repeat and its C-terminal C2 domain is sufficient for in vivo function. The DM14 domain forms a helical hairpin with a conserved, positively charged tip, that, in the structure of a DM14 domain-Shrub complex, occupies a negatively charged surface of Shrub that is otherwise used for homopolymerization. Lgd mutations at this interface disrupt its function in flies, confirming functional importance. Together, these data argue that Lgd regulates ESCRT activity by controlling access to the Shrub self-assembly surface.
Structural Basis for Regulation of ESCRT-III Complexes by Lgd.,McMillan BJ, Tibbe C, Drabek AA, Seegar TCM, Blacklow SC, Klein T Cell Rep. 2017 May 30;19(9):1750-1757. doi: 10.1016/j.celrep.2017.05.026. PMID:28564595[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Klein T. The tumour suppressor gene l(2)giant discs is required to restrict the activity of Notch to the dorsoventral boundary during Drosophila wing development. Dev Biol. 2003 Mar 15;255(2):313-33. PMID:12648493
- ↑ Childress JL, Acar M, Tao C, Halder G. Lethal giant discs, a novel C2-domain protein, restricts notch activation during endocytosis. Curr Biol. 2006 Nov 21;16(22):2228-33. Epub 2006 Nov 9. PMID:17088062 doi:http://dx.doi.org/S0960-9822(06)02224-X
- ↑ McMillan BJ, Tibbe C, Drabek AA, Seegar TCM, Blacklow SC, Klein T. Structural Basis for Regulation of ESCRT-III Complexes by Lgd. Cell Rep. 2017 May 30;19(9):1750-1757. doi: 10.1016/j.celrep.2017.05.026. PMID:28564595 doi:http://dx.doi.org/10.1016/j.celrep.2017.05.026
|
