2b3t

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[[Image:2b3t.gif|left|200px]]
 
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{{Structure
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==Structure of complex between E. coli translation termination factor RF1 and the PrmC methyltransferase==
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|PDB= 2b3t |SIZE=350|CAPTION= <scene name='initialview01'>2b3t</scene>, resolution 3.100&Aring;
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<StructureSection load='2b3t' size='340' side='right'caption='[[2b3t]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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<table><tr><td colspan='2'>[[2b3t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3T FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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|GENE= hemK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), prfA, sueB, uar ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3t OCA], [https://pdbe.org/2b3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3t RCSB], [https://www.ebi.ac.uk/pdbsum/2b3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3t ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1ml5|1ML5]], [[1gqe|1GQE]], [[1nv8|1NV8]], [[1t43|1T43]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3t OCA], [http://www.ebi.ac.uk/pdbsum/2b3t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b3t RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PRMC_ECOLI PRMC_ECOLI] Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif, i.e. on 'Gln-235' in RF1 and on 'Gln-252' in RF2.<ref>PMID:11805295</ref> <ref>PMID:11847124</ref> <ref>PMID:16364916</ref>
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== Evolutionary Conservation ==
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'''Molecular basis for bacterial class 1 release factor methylation by PrmC'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3t_consurf.spt"</scriptWhenChecked>
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Class I release factors bind to ribosomes in response to stop codons and trigger peptidyl-tRNA hydrolysis at the P site. Prokaryotic and eukaryotic RFs share one motif: a GGQ tripeptide positioned in a loop at the end of a stem region that interacts with the ribosomal peptidyl transferase center. The glutamine side chain of this motif is specifically methylated in both prokaryotes and eukaryotes. Methylation in E. coli is due to PrmC and results in strong stimulation of peptide chain release. We have solved the crystal structure of the complex between E. coli RF1 and PrmC bound to the methyl donor product AdoHCy. Both the GGQ domain (domain 3) and the central region (domains 2 and 4) of RF1 interact with PrmC. Structural and mutagenic data indicate a compact conformation of RF1 that is unlike its conformation when it is bound to the ribosome but is similar to the crystal structure of the protein alone.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2B3T is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3T OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3t ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Molecular basis for bacterial class I release factor methylation by PrmC., Graille M, Heurgue-Hamard V, Champ S, Mora L, Scrima N, Ulryck N, van Tilbeurgh H, Buckingham RH, Mol Cell. 2005 Dec 22;20(6):917-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16364916 16364916]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Buckingham, R H.]]
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[[Category: Buckingham RH]]
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[[Category: Champ, S.]]
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[[Category: Champ S]]
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[[Category: Graille, M.]]
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[[Category: Graille M]]
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[[Category: Heurgue-Hamard, V.]]
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[[Category: Heurgue-Hamard V]]
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[[Category: Mora, L.]]
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[[Category: Mora L]]
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[[Category: Scrima, N.]]
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[[Category: Scrima N]]
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[[Category: Tilbeurgh, H van.]]
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[[Category: Ulryck N]]
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[[Category: Ulryck, N.]]
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[[Category: Van Tilbeurgh H]]
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[[Category: conformational change]]
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[[Category: methylation]]
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[[Category: release factor]]
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[[Category: translation termination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:01:03 2008''
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Current revision

Structure of complex between E. coli translation termination factor RF1 and the PrmC methyltransferase

PDB ID 2b3t

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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