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| | ==Solution structure of NDP52 ubiquitin-binding zinc finger== | | ==Solution structure of NDP52 ubiquitin-binding zinc finger== |
| - | <StructureSection load='2mxp' size='340' side='right' caption='[[2mxp]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mxp' size='340' side='right'caption='[[2mxp]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mxp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MXP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MXP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mxp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MXP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xkl|4xkl]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CALCOCO2, NDP52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mxp OCA], [https://pdbe.org/2mxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mxp RCSB], [https://www.ebi.ac.uk/pdbsum/2mxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mxp ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mxp OCA], [http://pdbe.org/2mxp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mxp RCSB], [http://www.ebi.ac.uk/pdbsum/2mxp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mxp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CACO2_HUMAN CACO2_HUMAN]] May play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion.<ref>PMID:17635994</ref> | + | [https://www.uniprot.org/uniprot/CACO2_HUMAN CACO2_HUMAN] May play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion.<ref>PMID:17635994</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Pan, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Xie, X]] | + | [[Category: Pan L]] |
| - | [[Category: C2h2-type]] | + | [[Category: Xie X]] |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Ndp52]]
| + | |
| - | [[Category: Ubiquitin-binding]]
| + | |
| - | [[Category: Zinc finger]]
| + | |
| Structural highlights
Function
CACO2_HUMAN May play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion.[1]
Publication Abstract from PubMed
The autophagy receptor CALCOCO2/NDP52 functions as a bridging adaptor and plays an essential role in the selective autophagic degradation of invading pathogens by specifically recognizing ubiquitin-coated intracellular pathogens and subsequently targeting them to the autophagic machinery; thereby it is required for innate immune defense against a range of infectious pathogens in mammals. However, the mechanistic basis underlying CALCOCO2-mediated specific recognition of ubiqutinated pathogens is still unknown. Here, using biochemical and structural analyses, we demonstrated that the cargo-binding region of CALCOCO2 contains a dynamic unconventional zinc finger as well as a C2H2-type zinc-finger, and only the C2H2-type zinc finger specifically recognizes mono-ubiquitin or poly-ubiquitin chains. In addition to elucidating the specific ubiquitin recognition mechanism of CALCOCO2, the structure of the CALCOCO2 C2H2-type zinc finger in complex with mono-ubiquitin also uncovers a unique zinc finger-binding mode for ubiquitin. Our findings provide mechanistic insight into how CALCOCO2 targets ubiquitin-decorated pathogens for autophagic degradations.
Molecular basis of ubiquitin recognition by the autophagy receptor CALCOCO2.,Xie X, Li F, Wang Y, Wang Y, Lin Z, Cheng X, Liu J, Chen C, Pan L Autophagy. 2015;11(10):1775-89. doi: 10.1080/15548627.2015.1082025. PMID:26506893[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morriswood B, Ryzhakov G, Puri C, Arden SD, Roberts R, Dendrou C, Kendrick-Jones J, Buss F. T6BP and NDP52 are myosin VI binding partners with potential roles in cytokine signalling and cell adhesion. J Cell Sci. 2007 Aug 1;120(Pt 15):2574-85. Epub 2007 Jul 17. PMID:17635994 doi:jcs.007005
- ↑ Xie X, Li F, Wang Y, Wang Y, Lin Z, Cheng X, Liu J, Chen C, Pan L. Molecular basis of ubiquitin recognition by the autophagy receptor CALCOCO2. Autophagy. 2015;11(10):1775-89. doi: 10.1080/15548627.2015.1082025. PMID:26506893 doi:http://dx.doi.org/10.1080/15548627.2015.1082025
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