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| | ==Solution NMR structure of a novel cysteine framework containing Conus peptide Mo3964== | | ==Solution NMR structure of a novel cysteine framework containing Conus peptide Mo3964== |
| - | <StructureSection load='2mw7' size='340' side='right' caption='[[2mw7]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mw7' size='340' side='right'caption='[[2mw7]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mw7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Conmo Conmo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MW7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MW7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mw7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_monile Conus monile]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MW7 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mw7 OCA], [http://pdbe.org/2mw7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mw7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mw7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mw7 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mw7 OCA], [https://pdbe.org/2mw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mw7 RCSB], [https://www.ebi.ac.uk/pdbsum/2mw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mw7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CXRA_CONMO CXRA_CONMO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Conmo]] | |
| - | [[Category: Kancherla, A]] | |
| - | [[Category: Sarma, S P]] | |
| - | [[Category: Animal toxin]] | |
| - | [[Category: Conotoxin]] | |
| | [[Category: Conus monile]] | | [[Category: Conus monile]] |
| - | [[Category: Disulfide bond connectivity]] | + | [[Category: Large Structures]] |
| - | [[Category: Heteronuclear solution nmr spectroscopy]] | + | [[Category: Kancherla A]] |
| - | [[Category: Hydrogen bond]] | + | [[Category: Sarma SP]] |
| - | [[Category: M-superfamily]]
| + | |
| - | [[Category: Marine cone snail]]
| + | |
| - | [[Category: Neuronal ion-channel modulator]]
| + | |
| - | [[Category: Neuronal voltage-gated ion-channel modulator]]
| + | |
| - | [[Category: Peptide conformation]]
| + | |
| - | [[Category: Peptide scaffold]]
| + | |
| - | [[Category: Side-chain dihedral angle]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
CXRA_CONMO
Publication Abstract from PubMed
The structure of a new cysteine framework (-C-CC-C-C-C-) "M"-superfamily conotoxin, Mo3964, shows it to have a beta-sandwich structure that is stabilized by inter-sheet cross disulfide bonds. Mo3964 decreases outward K(+) currents in rat dorsal root ganglion neurons and increases the reversal potential of the NaV1.2 channels. The structure of Mo3964 (PDB ID: 2MW7 ) is constructed from the disulfide connectivity pattern, i.e., 1-3, 2-5, and 4-6, that is hitherto undescribed for the "M"-superfamily conotoxins. The tertiary structural fold has not been described for any of the known conus peptides. NOE (549), dihedral angle (84), and hydrogen bond (28) restraints, obtained by measurement of (h3)JNC' scalar couplings, were used as input for structure calculation. The ensemble of structures showed a backbone root mean square deviation of 0.68 +/- 0.18 A, with 87% and 13% of the backbone dihedral (varphi, psi) angles lying in the most favored and additional allowed regions of the Ramachandran map. The conotoxin Mo3964 represents a new bioactive peptide fold that is stabilized by disulfide bonds and adds to the existing repertoire of scaffolds that can be used to design stable bioactive peptide molecules.
A Disulfide Stabilized beta-Sandwich Defines the Structure of a New Cysteine Framework M-Superfamily Conotoxin.,Kancherla AK, Meesala S, Jorwal P, Palanisamy R, Sikdar SK, Sarma SP ACS Chem Biol. 2015 Aug 21;10(8):1847-60. doi: 10.1021/acschembio.5b00226. Epub, 2015 May 18. PMID:25961405[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kancherla AK, Meesala S, Jorwal P, Palanisamy R, Sikdar SK, Sarma SP. A Disulfide Stabilized beta-Sandwich Defines the Structure of a New Cysteine Framework M-Superfamily Conotoxin. ACS Chem Biol. 2015 Aug 21;10(8):1847-60. doi: 10.1021/acschembio.5b00226. Epub, 2015 May 18. PMID:25961405 doi:http://dx.doi.org/10.1021/acschembio.5b00226
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