5dm0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "5dm0" [edit=sysop:move=sysop])
Current revision (12:25, 6 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Crystal structure of the plantazolicin methyltransferase BamL in complex with triazolic desmethylPZN analog and SAH==
==Crystal structure of the plantazolicin methyltransferase BamL in complex with triazolic desmethylPZN analog and SAH==
-
<StructureSection load='5dm0' size='340' side='right' caption='[[5dm0]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
+
<StructureSection load='5dm0' size='340' side='right'caption='[[5dm0]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5dm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacvz Bacvz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DM0 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5dm0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_velezensis_FZB42 Bacillus velezensis FZB42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DM0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DM0 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5D8:ETHYL+2-(2-{2-[(1S)-1-AMINO-4-CARBAMIMIDAMIDOBUTYL]-1,3-THIAZOL-4-YL}-5-METHYL-1,3-OXAZOL-4-YL)-1,3-THIAZOLE-4-CARBOXYLATE'>5D8</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5dly|5dly]], [[5dm1|5dm1]], [[5dm2|5dm2]], [[5dm4|5dm4]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5D8:ETHYL+2-(2-{2-[(1S)-1-AMINO-4-CARBAMIMIDAMIDOBUTYL]-1,3-THIAZOL-4-YL}-5-METHYL-1,3-OXAZOL-4-YL)-1,3-THIAZOLE-4-CARBOXYLATE'>5D8</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RBAM_007500 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=326423 BACVZ])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dm0 OCA], [https://pdbe.org/5dm0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dm0 RCSB], [https://www.ebi.ac.uk/pdbsum/5dm0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dm0 ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dm0 OCA], [http://pdbe.org/5dm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dm0 RCSB], [http://www.ebi.ac.uk/pdbsum/5dm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dm0 ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A7Z2A9_BACVZ A7Z2A9_BACVZ]
-
Peptide antibiotics represent a class of conformationally constrained natural products of growing pharmaceutical interest. Plantazolicin (PZN) is a linear, polyheterocyclic natural product with highly selective and potent activity against the anthrax-causing bacterium, Bacillus anthracis. The bioactivity of PZN is contingent on dimethylation of its N-terminal Arg residue by an S-adenosylmethionine-dependent methyltransferase. Here, we explore the substrate tolerances of two homologous PZN methyltransferases by carrying out kinetic analyses of the enzymes against a synthetic panel of truncated PZN analogs containing the N-terminal Arg residue. X-ray cocrystal structures of the PZN methyltransferases with each of these heterocycle-containing substrates provide a rationale for understanding the strict substrate specificity of these enzymes. Kinetic studies of structure-guided, site-specific variants allowed for the assignment of residues governing catalysis and substrate scope. Microbiological testing further revealed that upon dimethylation of the N-terminal Arg, a pentaheterocyclized PZN analog retained potent anti-B. anthracis activity, nearly equal to that of full-length PZN. These studies may be useful in the biosynthetic engineering of natural product analogs with different bioactivity profiles, as demonstrated by our identification of a truncated plantazolicin derivative that is active against methicillin-resistant Staphylococcus aureus (MRSA).
+
-
 
+
-
Insights into methyltransferase specificity and bioactivity of derivatives of the antibiotic plantazolicin.,Hao Y, Blair PM, Sharma A, Mitchell DA, Nair SK ACS Chem Biol. 2015 May 15;10(5):1209-1216. doi: 10.1021/cb501042a. Epub 2015 Feb, 11. PMID:25635336<ref>PMID:25635336</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5dm0" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bacvz]]
+
[[Category: Bacillus velezensis FZB42]]
-
[[Category: Hao, Y]]
+
[[Category: Large Structures]]
-
[[Category: Nair, S K]]
+
[[Category: Hao Y]]
-
[[Category: Desmethylpzn analog]]
+
[[Category: Nair SK]]
-
[[Category: N-methyltransferase]]
+
-
[[Category: Plantazolicin]]
+
-
[[Category: Transferase-transferase inhibitor complex]]
+

Current revision

Crystal structure of the plantazolicin methyltransferase BamL in complex with triazolic desmethylPZN analog and SAH

PDB ID 5dm0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5dm0"
Personal tools