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| | ==Tom1 negatively modulates binding of Tollip to phosphatidylinositol 3-phosphate via a coupled folding and binding mechanism== | | ==Tom1 negatively modulates binding of Tollip to phosphatidylinositol 3-phosphate via a coupled folding and binding mechanism== |
| - | <StructureSection load='2n31' size='340' side='right' caption='[[2n31]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2n31' size='340' side='right'caption='[[2n31]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n31]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N31 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n31]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N31 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n31 OCA], [http://pdbe.org/2n31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n31 RCSB], [http://www.ebi.ac.uk/pdbsum/2n31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n31 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n31 OCA], [https://pdbe.org/2n31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n31 RCSB], [https://www.ebi.ac.uk/pdbsum/2n31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n31 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TOLIP_HUMAN TOLIP_HUMAN] Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.<ref>PMID:11751856</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Armstrong, G]] | + | [[Category: Large Structures]] |
| - | [[Category: Capelluto, D]] | + | [[Category: Armstrong G]] |
| - | [[Category: Xiao, S]] | + | [[Category: Capelluto D]] |
| - | [[Category: Immune system]] | + | [[Category: Xiao S]] |
| Structural highlights
Function
TOLIP_HUMAN Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.[1]
Publication Abstract from PubMed
Early endosomes represent the first sorting station for vesicular ubiquitylated cargo. Tollip, through its C2 domain, associates with endosomal phosphatidylinositol 3-phosphate (PtdIns(3)P) and binds ubiquitylated cargo in these compartments via its C2 and CUE domains. Tom1, through its GAT domain, is recruited to endosomes by binding to the Tollip Tom1-binding domain (TBD) through an unknown mechanism. Nuclear magnetic resonance data revealed that Tollip TBD is a natively unfolded domain that partially folds at its N terminus when bound to Tom1 GAT through high-affinity hydrophobic contacts. Furthermore, this association abrogates binding of Tollip to PtdIns(3)P by additionally targeting its C2 domain. Tom1 GAT is also able to bind ubiquitin and PtdIns(3)P at overlapping sites, albeit with modest affinity. We propose that association with Tom1 favors the release of Tollip from endosomal membranes, allowing Tollip to commit to cargo trafficking.
Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism.,Xiao S, Brannon MK, Zhao X, Fread KI, Ellena JF, Bushweller JH, Finkielstein CV, Armstrong GS, Capelluto DG Structure. 2015 Oct 6;23(10):1910-20. doi: 10.1016/j.str.2015.07.017. Epub 2015, Aug 27. PMID:26320582[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang G, Ghosh S. Negative regulation of toll-like receptor-mediated signaling by Tollip. J Biol Chem. 2002 Mar 1;277(9):7059-65. Epub 2001 Dec 18. PMID:11751856 doi:http://dx.doi.org/10.1074/jbc.M109537200
- ↑ Xiao S, Brannon MK, Zhao X, Fread KI, Ellena JF, Bushweller JH, Finkielstein CV, Armstrong GS, Capelluto DG. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Structure. 2015 Oct 6;23(10):1910-20. doi: 10.1016/j.str.2015.07.017. Epub 2015, Aug 27. PMID:26320582 doi:http://dx.doi.org/10.1016/j.str.2015.07.017
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