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| | ==Diversity in the structures and ligand binding sites among the fatty acid and retinol binding proteins of nematodes revealed by Na-FAR-1 from Necator americanus== | | ==Diversity in the structures and ligand binding sites among the fatty acid and retinol binding proteins of nematodes revealed by Na-FAR-1 from Necator americanus== |
| - | <StructureSection load='4uet' size='340' side='right' caption='[[4uet]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='4uet' size='340' side='right'caption='[[4uet]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4uet]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_hookworm Human hookworm]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UET FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4uet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Necator_americanus Necator americanus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UET FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uet OCA], [http://pdbe.org/4uet PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uet RCSB], [http://www.ebi.ac.uk/pdbsum/4uet PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uet ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uet OCA], [https://pdbe.org/4uet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uet RCSB], [https://www.ebi.ac.uk/pdbsum/4uet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uet ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/W2SRJ3_NECAM W2SRJ3_NECAM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4uet" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4uet" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Retinol-binding protein 3D structures|Retinol-binding protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human hookworm]] | + | [[Category: Large Structures]] |
| - | [[Category: Cooper, A]] | + | [[Category: Necator americanus]] |
| - | [[Category: Corsico, B]] | + | [[Category: Cooper A]] |
| - | [[Category: Griffiths, K]] | + | [[Category: Corsico B]] |
| - | [[Category: Kennedy, M W]] | + | [[Category: Griffiths K]] |
| - | [[Category: Rey-Burusco, M F]]
| + | [[Category: Ibanez Shimabukuro M]] |
| - | [[Category: Shimabukuro, M Ibanez]] | + | [[Category: Kennedy MW]] |
| - | [[Category: Smith, B O]] | + | [[Category: Rey-Burusco MF]] |
| - | [[Category: All-alpha]] | + | [[Category: Smith BO]] |
| - | [[Category: Fatty acid binding]] | + | |
| - | [[Category: Retinol binding]]
| + | |
| - | [[Category: Retinol-binding protein]]
| + | |
| Structural highlights
Function
W2SRJ3_NECAM
Publication Abstract from PubMed
Fatty acid and retinol-binding proteins (FARs) comprise a family of unusual alpha-helix rich lipid-binding proteins found exclusively in nematodes. They are secreted into host tissues by parasites of plants, animals and humans. The structure of a FAR protein from the free-living nematode Caenorhabditis elegans is available, but this protein [C. elegans FAR-7 (Ce-FAR-7)] is from a subfamily of FARs that does not appear to be important at the host/parasite interface. We have therefore examined [Necator americanus FAR-1 (Na-FAR-1)] from the blood-feeding intestinal parasite of humans, N. americanus. The 3D structure of Na-FAR-1 in its ligand-free and ligand-bound forms, determined by NMR (nuclear magnetic resonance) spectroscopy and X-ray crystallography respectively, reveals an alpha-helical fold similar to Ce-FAR-7, but Na-FAR-1 possesses a larger and more complex internal ligand-binding cavity and an additional C-terminal alpha-helix. Titration of apo-Na-FAR-1 with oleic acid, analysed by NMR chemical shift perturbation, reveals that at least four distinct protein-ligand complexes can be formed. Na-FAR-1 and possibly other FARs may have a wider repertoire for hydrophobic ligand binding, as confirmed in the present study by our finding that a range of neutral and polar lipids co-purify with the bacterially expressed recombinant protein. Finally, we show by immunohistochemistry that Na-FAR-1 is present in adult worms with a tissue distribution indicative of possible roles in nutrient acquisition by the parasite and in reproduction in the male.
Diversity in the structures and ligand-binding sites of nematode fatty acid and retinol-binding proteins revealed by Na-FAR-1 from Necator americanus.,Rey-Burusco MF, Ibanez-Shimabukuro M, Gabrielsen M, Franchini GR, Roe AJ, Griffiths K, Zhan B, Cooper A, Kennedy MW, Corsico B, Smith BO Biochem J. 2015 Nov 1;471(3):403-14. doi: 10.1042/BJ20150068. Epub 2015 Aug 28. PMID:26318523[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rey-Burusco MF, Ibanez-Shimabukuro M, Gabrielsen M, Franchini GR, Roe AJ, Griffiths K, Zhan B, Cooper A, Kennedy MW, Corsico B, Smith BO. Diversity in the structures and ligand-binding sites of nematode fatty acid and retinol-binding proteins revealed by Na-FAR-1 from Necator americanus. Biochem J. 2015 Nov 1;471(3):403-14. doi: 10.1042/BJ20150068. Epub 2015 Aug 28. PMID:26318523 doi:http://dx.doi.org/10.1042/BJ20150068
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