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4uet

From Proteopedia

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Diversity in the structures and ligand binding sites among the fatty acid and retinol binding proteins of nematodes revealed by Na-FAR-1 from Necator americanus

Structural highlights

4uet is a 1 chain structure with sequence from Necator americanus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

W2SRJ3_NECAM

Publication Abstract from PubMed

Fatty acid and retinol-binding proteins (FARs) comprise a family of unusual alpha-helix rich lipid-binding proteins found exclusively in nematodes. They are secreted into host tissues by parasites of plants, animals and humans. The structure of a FAR protein from the free-living nematode Caenorhabditis elegans is available, but this protein [C. elegans FAR-7 (Ce-FAR-7)] is from a subfamily of FARs that does not appear to be important at the host/parasite interface. We have therefore examined [Necator americanus FAR-1 (Na-FAR-1)] from the blood-feeding intestinal parasite of humans, N. americanus. The 3D structure of Na-FAR-1 in its ligand-free and ligand-bound forms, determined by NMR (nuclear magnetic resonance) spectroscopy and X-ray crystallography respectively, reveals an alpha-helical fold similar to Ce-FAR-7, but Na-FAR-1 possesses a larger and more complex internal ligand-binding cavity and an additional C-terminal alpha-helix. Titration of apo-Na-FAR-1 with oleic acid, analysed by NMR chemical shift perturbation, reveals that at least four distinct protein-ligand complexes can be formed. Na-FAR-1 and possibly other FARs may have a wider repertoire for hydrophobic ligand binding, as confirmed in the present study by our finding that a range of neutral and polar lipids co-purify with the bacterially expressed recombinant protein. Finally, we show by immunohistochemistry that Na-FAR-1 is present in adult worms with a tissue distribution indicative of possible roles in nutrient acquisition by the parasite and in reproduction in the male.

Diversity in the structures and ligand-binding sites of nematode fatty acid and retinol-binding proteins revealed by Na-FAR-1 from Necator americanus.,Rey-Burusco MF, Ibanez-Shimabukuro M, Gabrielsen M, Franchini GR, Roe AJ, Griffiths K, Zhan B, Cooper A, Kennedy MW, Corsico B, Smith BO Biochem J. 2015 Nov 1;471(3):403-14. doi: 10.1042/BJ20150068. Epub 2015 Aug 28. PMID:26318523^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rey-Burusco MF, Ibanez-Shimabukuro M, Gabrielsen M, Franchini GR, Roe AJ, Griffiths K, Zhan B, Cooper A, Kennedy MW, Corsico B, Smith BO. Diversity in the structures and ligand-binding sites of nematode fatty acid and retinol-binding proteins revealed by Na-FAR-1 from Necator americanus. Biochem J. 2015 Nov 1;471(3):403-14. doi: 10.1042/BJ20150068. Epub 2015 Aug 28. PMID:26318523 doi:http://dx.doi.org/10.1042/BJ20150068

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4uet"

@@ Line 1: / Line 1: @@
 ==Diversity in the structures and ligand binding sites among the fatty acid and retinol binding proteins of nematodes revealed by Na-FAR-1 from Necator americanus==
-<StructureSection load='4uet' size='340' side='right' caption='[[4uet]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='4uet' size='340' side='right'caption='[[4uet]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4uet]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_hookworm Human hookworm]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UET FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4uet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Necator_americanus Necator americanus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UET FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uet OCA], [http://pdbe.org/4uet PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uet RCSB], [http://www.ebi.ac.uk/pdbsum/4uet PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uet ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uet OCA], [https://pdbe.org/4uet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uet RCSB], [https://www.ebi.ac.uk/pdbsum/4uet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uet ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/W2SRJ3_NECAM W2SRJ3_NECAM]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 17: @@
 </div>
 <div class="pdbe-citations 4uet" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Retinol-binding protein 3D structures|Retinol-binding protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human hookworm]]
+[[Category: Large Structures]]
-[[Category: Cooper, A]]
+[[Category: Necator americanus]]
-[[Category: Corsico, B]]
+[[Category: Cooper A]]
-[[Category: Griffiths, K]]
+[[Category: Corsico B]]
-[[Category: Kennedy, M W]]
+[[Category: Griffiths K]]
-[[Category: Rey-Burusco, M F]]
+[[Category: Ibanez Shimabukuro M]]
-[[Category: Shimabukuro, M Ibanez]]
+[[Category: Kennedy MW]]
-[[Category: Smith, B O]]
+[[Category: Rey-Burusco MF]]
-[[Category: All-alpha]]
+[[Category: Smith BO]]
-[[Category: Fatty acid binding]]
-[[Category: Retinol binding]]
-[[Category: Retinol-binding protein]]

4uet

From Proteopedia

Current revision

Diversity in the structures and ligand binding sites among the fatty acid and retinol binding proteins of nematodes revealed by Na-FAR-1 from Necator americanus

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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