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| | ==Crystal structure of 34 kDa F-actin bundling protein from Dictyostelium discoideum== | | ==Crystal structure of 34 kDa F-actin bundling protein from Dictyostelium discoideum== |
| - | <StructureSection load='4x3n' size='340' side='right' caption='[[4x3n]], [[Resolution|resolution]] 1.89Å' scene=''> | + | <StructureSection load='4x3n' size='340' side='right'caption='[[4x3n]], [[Resolution|resolution]] 1.89Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4x3n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X3N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4x3n]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X3N FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">abpB, DDB_G0279081 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x3n OCA], [http://pdbe.org/4x3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x3n RCSB], [http://www.ebi.ac.uk/pdbsum/4x3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x3n ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x3n OCA], [https://pdbe.org/4x3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x3n RCSB], [https://www.ebi.ac.uk/pdbsum/4x3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x3n ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACTB_DICDI ACTB_DICDI]] May contribute to the structure and reorganization of filopodia and pseudopodia accompanying cell movements. | + | [https://www.uniprot.org/uniprot/ACTB_DICDI ACTB_DICDI] May contribute to the structure and reorganization of filopodia and pseudopodia accompanying cell movements. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The crystal structure of the 34 kDa F-actin-bundling protein ABP34 from Dictyostelium discoideum was solved by Ca(2+)/S-SAD phasing and refined at 1.89 A resolution. ABP34 is a calcium-regulated actin-binding protein that cross-links actin filaments into bundles. Its in vitro F-actin-binding and F-actin-bundling activities were confirmed by a co-sedimentation assay and transmission electron microscopy. The co-localization of ABP34 with actin in cells was also verified. ABP34 adopts a two-domain structure with an EF-hand-containing N-domain and an actin-binding C-domain, but has no reported overall structural homologues. The EF-hand is occupied by a calcium ion with a pentagonal bipyramidal coordination as in the canonical EF-hand. The C-domain structure resembles a three-helical bundle and superposes well onto the rod-shaped helical structures of some cytoskeletal proteins. Residues 216-244 in the C-domain form part of the strongest actin-binding sites (193-254) and exhibit a conserved sequence with the actin-binding region of alpha-actinin and ABP120. Furthermore, the second helical region of the C-domain is kinked by a proline break, offering a convex surface towards the solvent area which is implicated in actin binding. The F-actin-binding model suggests that ABP34 binds to the side of the actin filament and residues 216-244 fit into a pocket between actin subdomains -1 and -2 through hydrophobic interactions. These studies provide insights into the calcium coordination in the EF-hand and F-actin-binding site in the C-domain of ABP34, which are associated through interdomain interactions.
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| - | Structure of the 34 kDa F-actin-bundling protein ABP34 from Dictyostelium discoideum.,Kim MK, Kim JH, Kim JS, Kang SO Acta Crystallogr D Biol Crystallogr. 2015 Sep;71(Pt 9):1835-49. doi:, 10.1107/S139900471501264X. Epub 2015 Aug 25. PMID:26327373<ref>PMID:26327373</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4x3n" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 11735]] | + | [[Category: Dictyostelium discoideum]] |
| - | [[Category: Kang, S O]] | + | [[Category: Large Structures]] |
| - | [[Category: Kim, J H]] | + | [[Category: Kang S-O]] |
| - | [[Category: Kim, J S]] | + | [[Category: Kim J-H]] |
| - | [[Category: Kim, M K]] | + | [[Category: Kim J-S]] |
| - | [[Category: Actin]]
| + | [[Category: Kim M-K]] |
| - | [[Category: Bundling]]
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| - | [[Category: Cytoskeleton]]
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| - | [[Category: Protein binding]]
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