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| | ==Structure of a phospholipid trafficking complex with substrate== | | ==Structure of a phospholipid trafficking complex with substrate== |
| - | <StructureSection load='4xiz' size='340' side='right' caption='[[4xiz]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4xiz' size='340' side='right'caption='[[4xiz]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xiz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XIZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XIZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xiz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XIZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XIZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPP:2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL+HEXADECANOATE'>LPP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xhr|4xhr]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LPP:2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL+HEXADECANOATE'>LPP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UPS1, YLR193C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), MDM35, YKL053C-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xiz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xiz OCA], [https://pdbe.org/4xiz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xiz RCSB], [https://www.ebi.ac.uk/pdbsum/4xiz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xiz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xiz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xiz OCA], [http://pdbe.org/4xiz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xiz RCSB], [http://www.ebi.ac.uk/pdbsum/4xiz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xiz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UPS1_YEAST UPS1_YEAST]] Required for maintenance of normal mitochondrial morphology as well as PCP1-dependent processing of MGM1. With UPS2, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits.<ref>PMID:16754953</ref> <ref>PMID:19221197</ref> <ref>PMID:19506038</ref> <ref>PMID:20622808</ref> [[http://www.uniprot.org/uniprot/MDM35_YEAST MDM35_YEAST]] Involved in mitochondrial distribution and morphology. Mediates the import of UPS1, UPS2 and UPS3, 3 atypical mitochondrial intermembrane space (IMS) proteins lacking the two major IMS-targeting signals, into the intermembrane space.<ref>PMID:11907266</ref> <ref>PMID:20622808</ref> <ref>PMID:20657548</ref> | + | [https://www.uniprot.org/uniprot/UPS1_YEAST UPS1_YEAST] Required for maintenance of normal mitochondrial morphology as well as PCP1-dependent processing of MGM1. With UPS2, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits.<ref>PMID:16754953</ref> <ref>PMID:19221197</ref> <ref>PMID:19506038</ref> <ref>PMID:20622808</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: He, F]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Wang, C]] | + | [[Category: He F]] |
| - | [[Category: Yu, F]] | + | [[Category: Wang C]] |
| - | [[Category: Zhang, P]] | + | [[Category: Yu F]] |
| - | [[Category: Lipid transport-oxidoreductase complex]]
| + | [[Category: Zhang P]] |
| - | [[Category: Phospholipid]]
| + | |
| Structural highlights
Function
UPS1_YEAST Required for maintenance of normal mitochondrial morphology as well as PCP1-dependent processing of MGM1. With UPS2, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits.[1] [2] [3] [4]
Publication Abstract from PubMed
Ups1 forms a complex with Mdm35 and is critical for the transport of phosphatidic acid (PA) from the mitochondrial outer membrane to the inner membrane. We report the crystal structure of the Ups1-Mdm35-PA complex and the functional characterization of Ups1-Mdm35 in PA binding and transfer. Ups1 features a barrel-like structure consisting of an antiparallel beta-sheet and three alpha-helices. Mdm35 adopts a three-helical clamp-like structure to wrap around Ups1 to form a stable complex. The beta-sheet and alpha-helices of Ups1 form a long tunnel-like pocket to accommodate the substrate PA, and a short helix alpha2 acts as a lid to cover the pocket. The hydrophobic residues lining the pocket and helix alpha2 are critical for PA binding and transfer. In addition, a hydrophilic patch on the surface of Ups1 near the PA phosphate-binding site also plays an important role in the function of Ups1-Mdm35. Our study reveals the molecular basis of the function of Ups1-Mdm35 and sheds new light on the mechanism of intramitochondrial phospholipid transport by the MSF1/PRELI family proteins.
Structural basis of intramitochondrial phosphatidic acid transport mediated by Ups1-Mdm35 complex.,Yu F, He F, Yao H, Wang C, Wang J, Li J, Qi X, Xue H, Ding J, Zhang P EMBO Rep. 2015 Jun 12. pii: e201540137. PMID:26071601[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sesaki H, Dunn CD, Iijima M, Shepard KA, Yaffe MP, Machamer CE, Jensen RE. Ups1p, a conserved intermembrane space protein, regulates mitochondrial shape and alternative topogenesis of Mgm1p. J Cell Biol. 2006 Jun 5;173(5):651-8. PMID:16754953 doi:http://dx.doi.org/10.1083/jcb.200603092
- ↑ Osman C, Haag M, Potting C, Rodenfels J, Dip PV, Wieland FT, Brugger B, Westermann B, Langer T. The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria. J Cell Biol. 2009 Feb 23;184(4):583-96. doi: 10.1083/jcb.200810189. Epub 2009 Feb, 16. PMID:19221197 doi:http://dx.doi.org/10.1083/jcb.200810189
- ↑ Tamura Y, Endo T, Iijima M, Sesaki H. Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. J Cell Biol. 2009 Jun 15;185(6):1029-45. Epub 2009 Jun 8. PMID:19506038 doi:http://dx.doi.org/jcb.200812018
- ↑ Tamura Y, Iijima M, Sesaki H. Mdm35p imports Ups proteins into the mitochondrial intermembrane space by functional complex formation. EMBO J. 2010 Sep 1;29(17):2875-87. doi: 10.1038/emboj.2010.149. Epub 2010 Jul 9. PMID:20622808 doi:http://dx.doi.org/10.1038/emboj.2010.149
- ↑ Yu F, He F, Yao H, Wang C, Wang J, Li J, Qi X, Xue H, Ding J, Zhang P. Structural basis of intramitochondrial phosphatidic acid transport mediated by Ups1-Mdm35 complex. EMBO Rep. 2015 Jun 12. pii: e201540137. PMID:26071601 doi:http://dx.doi.org/10.15252/embr.201540137
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