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| | ==Crystal Structure of a Complex Between the SNARE Vam3 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum== | | ==Crystal Structure of a Complex Between the SNARE Vam3 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum== |
| - | <StructureSection load='5buz' size='340' side='right' caption='[[5buz]], [[Resolution|resolution]] 3.10Å' scene=''> | + | <StructureSection load='5buz' size='340' side='right'caption='[[5buz]], [[Resolution|resolution]] 3.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5buz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BUZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5buz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BUZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kmo|4kmo]], [[5bv1|5bv1]], [[5bv0|5bv0]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0057760 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0026760 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0015850 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5buz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5buz OCA], [https://pdbe.org/5buz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5buz RCSB], [https://www.ebi.ac.uk/pdbsum/5buz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5buz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5buz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5buz OCA], [http://pdbe.org/5buz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5buz RCSB], [http://www.ebi.ac.uk/pdbsum/5buz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5buz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G0SCM5_CHATD G0SCM5_CHATD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chatd]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| - | [[Category: Baker, R W]] | + | [[Category: Large Structures]] |
| - | [[Category: Hughson, F M]] | + | [[Category: Baker RW]] |
| - | [[Category: Jeffrey, P D]] | + | [[Category: Hughson FM]] |
| - | [[Category: Hops complex]] | + | [[Category: Jeffrey PD]] |
| - | [[Category: Membrane trafficking]]
| + | |
| - | [[Category: Sm protein]]
| + | |
| - | [[Category: Snare domain]]
| + | |
| - | [[Category: Thermophile]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
G0SCM5_CHATD
Publication Abstract from PubMed
Fusion of intracellular transport vesicles requires soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and Sec1/Munc18-family (SM) proteins. Membrane-bridging SNARE complexes are critical for fusion, but their spontaneous assembly is inefficient and may require SM proteins in vivo. We report x-ray structures of Vps33, the SM subunit of the yeast homotypic fusion and vacuole protein-sorting (HOPS) complex, bound to two individual SNAREs. The two SNAREs, one from each membrane, are held in the correct orientation and register for subsequent complex assembly. Vps33 and potentially other SM proteins could thus act as templates for generating partially zipped SNARE assembly intermediates. HOPS was essential to mediate SNARE complex assembly at physiological SNARE concentrations. Thus, Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition.
A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.,Baker RW, Jeffrey PD, Zick M, Phillips BP, Wickner WT, Hughson FM Science. 2015 Sep 4;349(6252):1111-4. doi: 10.1126/science.aac7906. PMID:26339030[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Baker RW, Jeffrey PD, Zick M, Phillips BP, Wickner WT, Hughson FM. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science. 2015 Sep 4;349(6252):1111-4. doi: 10.1126/science.aac7906. PMID:26339030 doi:http://dx.doi.org/10.1126/science.aac7906
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