2b9x

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[[Image:2b9x.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of CLA-producing fatty acid isomerase from P. acnes==
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|PDB= 2b9x |SIZE=350|CAPTION= <scene name='initialview01'>2b9x</scene>, resolution 2.22&Aring;
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<StructureSection load='2b9x' size='340' side='right'caption='[[2b9x]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[2b9x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cutibacterium_acnes Cutibacterium acnes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B9X FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b9x OCA], [https://pdbe.org/2b9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b9x RCSB], [https://www.ebi.ac.uk/pdbsum/2b9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b9x ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2b9w|2B9W]], [[2b9y|2B9Y]], [[2ba9|2BA9]], [[2bab|2BAB]], [[2bac|2BAC]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b9x OCA], [http://www.ebi.ac.uk/pdbsum/2b9x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b9x RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q6A8X5_CUTAK Q6A8X5_CUTAK]
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== Evolutionary Conservation ==
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'''Crystal Structure of CLA-producing fatty acid isomerase from P. acnes'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/2b9x_consurf.spt"</scriptWhenChecked>
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Conjugated linoleic acids (CLAs) affect body fat gain, carcinogenesis, insulin resistance, and lipid peroxidation in mammals. Several isomers of CLA exist, of which the (9Z, 11E) and (10E, 12Z) isomers have beneficial effects on human metabolism but are scarce in foods. Bacterial polyunsaturated fatty acid isomerases are promising biotechnological catalysts for CLA production. We describe six crystal structures of the Propionibacterium acnes polyunsaturated fatty acid isomerase PAI in apo- and product-bound forms. The three-domain flavoprotein has previously undescribed folds outside the FAD-binding site. Conformational changes in a hydrophobic channel toward the active site reveal a unique gating mechanism for substrate specificity. The geometry of the substrate-binding site explains the length preferences for C18 fatty acids. A catalytic mechanism for double-bond isomerization is formulated that may be altered to change substrate specificity for syntheses of rare CLAs from easily accessible precursors.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2B9X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Propionibacterium_acnes Propionibacterium acnes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9X OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b9x ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structure and mechanism of the Propionibacterium acnes polyunsaturated fatty acid isomerase., Liavonchanka A, Hornung E, Feussner I, Rudolph MG, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2576-81. Epub 2006 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16477020 16477020]
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</StructureSection>
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[[Category: Propionibacterium acnes]]
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[[Category: Cutibacterium acnes]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Liavonchanka, A.]]
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[[Category: Liavonchanka A]]
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[[Category: Rudolph, M G.]]
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[[Category: Rudolph MG]]
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[[Category: conjugated linoleic acid]]
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[[Category: fad]]
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[[Category: isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:03:22 2008''
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Current revision

Crystal Structure of CLA-producing fatty acid isomerase from P. acnes

PDB ID 2b9x

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