2bcw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:17, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2bcw.gif|left|200px]]
 
-
{{Structure
+
==Coordinates of the N-terminal domain of ribosomal protein L11,C-terminal domain of ribosomal protein L7/L12 and a portion of the G' domain of elongation factor G, as fitted into cryo-em map of an Escherichia coli 70S*EF-G*GDP*fusidic acid complex==
-
|PDB= 2bcw |SIZE=350|CAPTION= <scene name='initialview01'>2bcw</scene>
+
<SX load='2bcw' size='340' side='right' viewer='molstar' caption='[[2bcw]], [[Resolution|resolution]] 11.20&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[2bcw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] and [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BCW FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.2&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bcw OCA], [https://pdbe.org/2bcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bcw RCSB], [https://www.ebi.ac.uk/pdbsum/2bcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bcw ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=[[1mms|1MMS]], [[1ctf|1CTF]], [[1elo|1ELO]]
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bcw OCA], [http://www.ebi.ac.uk/pdbsum/2bcw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bcw RCSB]</span>
+
[https://www.uniprot.org/uniprot/RL7_ECOLI RL7_ECOLI] Seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation.[HAMAP-Rule:MF_00368]
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/2bcw_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bcw ConSurf].
 +
<div style="clear:both"></div>
-
'''Coordinates of the N-terminal domain of ribosomal protein L11,C-terminal domain of ribosomal protein L7/L12 and a portion of the G' domain of elongation factor G, as fitted into cryo-em map of an Escherichia coli 70S*EF-G*GDP*fusidic acid complex'''
+
==See Also==
-
 
+
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
-
 
+
*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
-
==Overview==
+
*[[Ribosomal protein L7/L12|Ribosomal protein L7/L12]]
-
During tRNA translocation on the ribosome, an arc-like connection (ALC) is formed between the G' domain of elongation factor G (EF-G) and the L7/L12-stalk base of the large ribosomal subunit in the GDP state. To delineate the boundary of EF-G within the ALC, we tagged an amino acid residue near the tip of the G' domain of EF-G with undecagold, which was then visualized with three-dimensional cryo-electron microscopy (cryo-EM). Two distinct positions for the undecagold, observed in the GTP-state and GDP-state cryo-EM maps of the ribosome bound EF-G, allowed us to determine the movement of the labeled amino acid. Molecular analyses of the cryo-EM maps show: (1) that three structural components, the N-terminal domain of ribosomal protein L11, the C-terminal domain of ribosomal protein L7/L12, and the G' domain of EF-G, participate in formation of the ALC; and (2) that both EF-G and the ribosomal protein L7/L12 undergo large conformational changes to form the ALC.
+
__TOC__
-
 
+
</SX>
-
==About this Structure==
+
-
2BCW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BCW OCA].
+
-
 
+
-
==Reference==
+
-
Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal protein L7/L12 during translocation as revealed by cryo-EM., Datta PP, Sharma MR, Qi L, Frank J, Agrawal RK, Mol Cell. 2005 Dec 9;20(5):723-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16337596 16337596]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
-
[[Category: Agrawal, R K.]]
+
[[Category: Agrawal RK]]
-
[[Category: Datta, P P.]]
+
[[Category: Datta PP]]
-
[[Category: Frank, J.]]
+
[[Category: Frank J]]
-
[[Category: Qi, L.]]
+
[[Category: Qi L]]
-
[[Category: Sharma, M R.]]
+
[[Category: Sharma MR]]
-
[[Category: components involved in interaction between ef-g and l7/l12 stalk base of the ribosome]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:04:37 2008''
+

Current revision

Coordinates of the N-terminal domain of ribosomal protein L11,C-terminal domain of ribosomal protein L7/L12 and a portion of the G' domain of elongation factor G, as fitted into cryo-em map of an Escherichia coli 70S*EF-G*GDP*fusidic acid complex

2bcw, resolution 11.20Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bcw"
Personal tools